| Separation of a protein factor necessary for the oxidative desaturation of fatty acids in the rat. | |
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MedLine Citation:
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PMID: 1165249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microsomes from rat liver were extracted by low ionic strength solutions. Extracted microsomes lost most of the linoleic acid desaturation activity. The addition of the extract back into the extracted microsomes was necessary to restore full desaturation activity. The soluble fraction had no desaturation activity. The existence of a soluble factor loosely bound to the microsomes, stable to sonication, and unstable to heat and trypsin digestion was recognized. This protein could not be replaced by albumin. The factor was also essential for the oxidative desaturation of palmitic, stearic, linoleic, and gamma-linolenic acid. The present experiment suggests that the protein factor is not NADH-cytochrome b5 reductase, cytochrome b5, or the cyanide-sensitive factor. |
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Authors:
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A Catalá; A M Nervi; R R Brenner |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 250 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1975 Sep |
Date Detail:
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Created Date: 1975-12-11 Completed Date: 1975-12-11 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7481-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cytochrome Reductases / metabolism Fatty Acid Desaturases / metabolism* Kinetics Male Microsomes, Liver / drug effects, enzymology* Proteins / physiology* Rats Structure-Activity Relationship Temperature |
| Chemical | |
Reg. No./Substance:
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0/Proteins; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.6.2.-/Cytochrome Reductases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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