Document Detail


Separation, pre-concentration, and HPLC analysis of methylxanthines in urine samples.
MedLine Citation:
PMID:  15537072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An SPE method, using RP18 phases, for the simultaneous extraction of caffeine, theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1,7-dimethyluric acid, and 1,3,7-trimethyluric acid from urine has been developed. Besides a gradient HPLC system for the analysis of the compounds of interest on a LiChrosorb RP-18 (7 microm) column with mobile phase containing 0.05% aq. solution of trifluoroacetic acid and acetonitrile has been elaborated. The procedure has been successfully applied to the analysis of methylxanthines and methyluric acids in urine of patients with chronic asthma treated with theophylline and in urine of healthy subjects.
Authors:
Mirosława Zydroń; Jacek Baranowski; Irena Baranowska
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of separation science     Volume:  27     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-11     Completed Date:  2005-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1166-72     Citation Subset:  IM    
Affiliation:
Department of Analytical and General Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland.
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MeSH Terms
Descriptor/Qualifier:
Caffeine / urine
Calibration
Chromatography, High Pressure Liquid / instrumentation,  methods*
Humans
Molecular Structure
Reproducibility of Results
Sensitivity and Specificity
Theobromine / urine
Uric Acid / urine
Urine / chemistry*
Xanthines / urine*
Chemical
Reg. No./Substance:
0/Xanthines; 28109-92-4/methylxanthine; 58-08-2/Caffeine; 69-93-2/Uric Acid; 83-67-0/Theobromine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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