| Separation of plant hormones from biofertilizer by capillary electrophoresis using a capillary coated dynamically with polycationic polymers. | |
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MedLine Citation:
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PMID: 16772676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new, simple and rapid capillary electrophoresis (CE) method, using hexadimethrine bromide (HDB) as electroosmotic flow (EOF) modifier, was developed for the identification and quantitative determination of four plant hormones, including gibberellin A3 (GA3), indole-3-acetic acid (IAA), alpha-naphthaleneacetic acid (NAA) and 4-chlorophenoxyacetic acid (4-CA). The optimum separation was achieved with 20 mM borate buffer at pH 10.00 containing 0.005% (w/v) of HDB. The applied voltage was -25 kV and the capillary temperature was kept constant at 25 degrees C. Salicylic acid was used as internal standard for quantification. The calibration dependencies exhibited good linearity within the ratios of the concentrations of standard samples and internal standard and the ratios of the peak areas of samples and internal standard. The correlation coefficients were from 0.9952 to 0.9997. The relative standard deviations of migration times and peak areas were < 1.93 and 6.84%, respectively. The effects of buffer pH, the concentration of HDB and the voltage on the resolution were studied systematically. By this method, the contents of plant hormone in biofertilizer were successfully determined within 7 min, with satisfactory repeatability and recovery. |
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Authors:
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Ting-Fu Jiang; Zhi-Hua Lv; Yuan-Hong Wang; Mei-E Yue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 22 ISSN: 0910-6340 ISO Abbreviation: Anal Sci Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-14 Completed Date: 2007-05-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 811-4 Citation Subset: IM |
Affiliation:
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Marine Drug and Food Institute, Ocean University of China, Qingdao, P. R. China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dichlorophenoxyacetic Acid
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analogs & derivatives,
chemistry Calibration Cations Electrophoresis, Capillary / methods* Fertilizers Gibberellins / chemistry Hexadimethrine / chemistry Hydrogen-Ion Concentration Indoleacetic Acids / chemistry Methanol / chemistry Models, Chemical Naphthaleneacetic Acids / chemistry Plant Growth Regulators / analysis*, chemistry Polymers / chemistry Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Cations; 0/Fertilizers; 0/Gibberellins; 0/Indoleacetic Acids; 0/Naphthaleneacetic Acids; 0/Plant Growth Regulators; 0/Polymers; 122-88-3/4-chlorophenoxyacetic acid; 67-56-1/Methanol; 77-06-5/gibberellic acid; 86-87-3/1-naphthaleneacetic acid; 87-51-4/indoleacetic acid; 9011-04-5/Hexadimethrine; 94-75-7/2,4-Dichlorophenoxyacetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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