Document Detail

Separation of murine neutrophils and macrophages by thermoresponsive magnetic nanoparticles.
MedLine Citation:
PMID:  17949107     Owner:  NLM     Status:  MEDLINE    
Magnetic particles have been used widely in both biotechnological and medical fields, including for immunoassay, enzyme immobilization, drug transport, and immunological diagnosis. Especially particles with bioactive molecules such as antibodies and streptavidin are very useful tools for cell separation. Here we report affinity selection of neutrophils and macrophages from peritoneal inflammatory cells performed by thermoresponsive magnetic nanoparticles conjugated with macrophage-specific anti-F4/80 antibody. The magnetic nanoparticles, which are capped with thermoresponsive polymers, are aggregated by heating the particles over 30 degrees C and show their intrinsic magnetism. The neutrophils are concentrated approximately 90% by these magnetic nanoparticles without any activation, indicating that this novel cell separation method could fulfill a wide range of applications in analysis of the isolation of fragile cells such as neutrophils.
Akiyoshi Hoshino; Noriyuki Ohnishi; Masato Yasuhara; Kenji Yamamoto; Akihiko Kondo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-20
Journal Detail:
Title:  Biotechnology progress     Volume:  23     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2007-12-07     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1513-6     Citation Subset:  IM    
International Clinical Research Center, Research Institute, International Medical Center of Japan, Tokyo, Japan.
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MeSH Terms
Flow Cytometry
Immunomagnetic Separation / methods*
Macrophages / cytology*
Mice, Inbred C57BL
Nanoparticles / chemistry*
Neutrophils / cytology*

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