| Separation of human apolipoproteins A-IV, A-I and E by reversed-phase high-performance liquid chromatography on a TSK Phenyl-5PW column. | |
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MedLine Citation:
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PMID: 2120276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method has been developed for the rapid separation of the medium-molecular-weight apolipoproteins A-IV, A-I and E by high-performance liquid chromatography. Separations were achieved using a commercially available column of very low hydrophobicity (TSK Phenyl-5PW) in the reversed-phase mode rather than the conventional mode of hydrophobic interaction. Delipidated apolipoproteins were dissolved in 20 mM orthophosphoric acid (pH 2.3), applied to the column which was pre-equilibrated with the same buffer, and eluted with an increasing gradient of acetonitrile. Purified apolipoproteins were identified by a combination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis, amino acid analysis and N-terminal sequence analysis. In one step the method can be used to separate the major human chylomicron apolipoproteins A-IV, A-I and E, following preliminary removal of apolipoprotein A-II and the C apolipoproteins by size-exclusion chromatography. |
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Authors:
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T Tetaz; E Kecorius; B Grego; N Fidge |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography Volume: 511 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1990 Jul |
Date Detail:
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Created Date: 1990-11-16 Completed Date: 1990-11-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 147-53 Citation Subset: IM |
Affiliation:
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Baker Medical Research Institute, Melbourne, Victoria, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis Apolipoprotein A-I Apolipoproteins A / isolation & purification* Apolipoproteins E / isolation & purification* Chromatography, High Pressure Liquid Chylomicrons / isolation & purification Electrophoresis, Polyacrylamide Gel Humans Indicators and Reagents Lymph / chemistry Molecular Sequence Data |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Apolipoprotein A-I; 0/Apolipoproteins A; 0/Apolipoproteins E; 0/Chylomicrons; 0/Indicators and Reagents; 0/apolipoprotein A-IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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