Document Detail


Separation of human apolipoproteins A-IV, A-I and E by reversed-phase high-performance liquid chromatography on a TSK Phenyl-5PW column.
MedLine Citation:
PMID:  2120276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method has been developed for the rapid separation of the medium-molecular-weight apolipoproteins A-IV, A-I and E by high-performance liquid chromatography. Separations were achieved using a commercially available column of very low hydrophobicity (TSK Phenyl-5PW) in the reversed-phase mode rather than the conventional mode of hydrophobic interaction. Delipidated apolipoproteins were dissolved in 20 mM orthophosphoric acid (pH 2.3), applied to the column which was pre-equilibrated with the same buffer, and eluted with an increasing gradient of acetonitrile. Purified apolipoproteins were identified by a combination of sodium dodecyl sulphate-polyacrylamide gel electrophoresis, amino acid analysis and N-terminal sequence analysis. In one step the method can be used to separate the major human chylomicron apolipoproteins A-IV, A-I and E, following preliminary removal of apolipoprotein A-II and the C apolipoproteins by size-exclusion chromatography.
Authors:
T Tetaz; E Kecorius; B Grego; N Fidge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography     Volume:  511     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-11-16     Completed Date:  1990-11-16     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  147-53     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Apolipoprotein A-I
Apolipoproteins A / isolation & purification*
Apolipoproteins E / isolation & purification*
Chromatography, High Pressure Liquid
Chylomicrons / isolation & purification
Electrophoresis, Polyacrylamide Gel
Humans
Indicators and Reagents
Lymph / chemistry
Molecular Sequence Data
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Apolipoprotein A-I; 0/Apolipoproteins A; 0/Apolipoproteins E; 0/Chylomicrons; 0/Indicators and Reagents; 0/apolipoprotein A-IV

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