Document Detail


Separation of homologues and isomers of linear alkylbenzenesulfonates by capillary electrophoresis with sodium dodecyl sulfate, carboxylic acids and bile salts.
MedLine Citation:
PMID:  12601738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of several anionic compounds, including carboxylic and dicarboxylic acids, sodium dodecyl sulfate (SDS), and sodium deoxycholate (SDC) and other bile salts, to separate the C(10)-C(13) homologues and the corresponding 20 positional isomers of linear alkylbenzenesulfonates (LAS) by capillary electrophoresis was studied. Up to 19 peaks and a shoulder were observed with a background electrolyte (BGE) containing 10 mM phosphate (pH 6.8), 30% acetonitrile and 40 mM SDS, and 18 peaks were obtained with a BGE containing 10 mM borate (pH 9), 40% ethanol and 40 mM palmitic acid (PA). Resolution increased with the alkyl chain length of the carboxylic acid. Dicarboxylic acids with a short alkyl chain, as azelaic acid, were useful to separate the homologues without distinguishing between the isomers. Up to 16 peaks and a shoulder were distinguished with SDC. Resolution decreased with the other bile salts. The 6-C(11)/5-C(11) isomer pair was better resolved with SDC than with SDS, and the 2-C(12) isomer was isolated using both PA and SDC, but not with SDS. Only the 7-C(13)/6-C(13) pair could not be resolved with any of the discriminating agents used.
Authors:
José Manuel Herrero-Martínez; Ernesto F Simó-Alfonso; Guillermo Ramis-Ramos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  24     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-25     Completed Date:  2003-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  681-6     Citation Subset:  IM    
Affiliation:
Departament de Química Analítica, Universitat de Barcelona, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / chemistry*
Carboxylic Acids / chemistry*
Electrophoresis, Capillary / methods*
Isomerism
Sodium Dodecyl Sulfate / chemistry*
Sulfonic Acids / chemistry,  isolation & purification*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Carboxylic Acids; 0/Sulfonic Acids; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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