| Separation of gamma-aminobutyric acid from fermented broth. | |
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MedLine Citation:
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PMID: 21614609 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Gamma-aminobutyric acid (GABA) is a non-proteinaceous amino acid that is widely distributed in nature and acts as the major inhibitory neurotransmitter in the mammalian brain. This study aimed to find a separation method for getting high-purity GABA from a fermented broth. Firstly, a fermented broth with a high content of GABA (reaching 997 ± 51 mM) was prepared by fermentation with Lactobacillus brevis NCL912. GABA purification was conducted by successive centrifugation, filtration, decoloration, desalination, ion-exchange chromatography (IEC), and crystallization. Inorganic salt (Na(2)SO(4)) was removed from the both by desalination with 70% ethanol solution. A ninhydrin test strip was designed for the real-time detection of GABA during IEC. The recovery rate for the whole purification process was about 50%. The purified product was characterized by thin-layer chromatography and HPLC, and its purity reached 98.66 ± 2.36%. |
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Authors:
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Haixing Li; Ting Qiu; Yan Chen; Yusheng Cao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-26 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: - ISSN: 1476-5535 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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State Key Laboratory of Food Science and Technology, Nanchang University, 330047, Nanchang, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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