| Separation and determination of some stereoisomers by capillary gel electrophoresis with cyclodextrin incorporated in polyacrylamide gel. | |
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MedLine Citation:
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PMID: 15048432 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Capillary gel electrophoresis (CGE) was successfully applied to the separation of optically active isomers and position isomers by incorporating a suitable cyclodextrin chiral selector in polyacrylamide gel. A commercially available ss-cyclodextrin (ss-CD) was used for enantioselectivity towards o-, m- and p-nitrobenzoic acid, o-, m- and p-hydroxybenzoic acid, o-, m- and p-toluic acid and the optical isomers of dansyl-D,L-leucine and R,S-1,1'-binaphthyl-2,2-dihydrogenphosphate. Especially the effect of organic solvents, such as acetonitrile, methanol, dimethylsulphoxide and others were examined in detail. The resolution varied to some extent with the addition of the organic solvent to the polyacrylamide gel and the running buffer solution. The possible mechanism has also been discussed. In addition, quantitative aspects of the separation of stereoisomers using CGE have been studied, showing that both the resolution and accuracy of the determinations were affected by the ratio of the enantiomers. |
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Authors:
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J M Lin; T Nakagama; H Okazawa; X Z Wu; T Hobo |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 354 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 1996 Feb |
Date Detail:
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Created Date: 2004-03-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 451-4 Citation Subset: - |
Affiliation:
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Department of Industrial Chemistry, Faculty of Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, 192-03, Hachioji, Tokyo, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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