Document Detail


Separation and determination of some stereoisomers by capillary gel electrophoresis with cyclodextrin incorporated in polyacrylamide gel.
MedLine Citation:
PMID:  15048432     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Capillary gel electrophoresis (CGE) was successfully applied to the separation of optically active isomers and position isomers by incorporating a suitable cyclodextrin chiral selector in polyacrylamide gel. A commercially available ss-cyclodextrin (ss-CD) was used for enantioselectivity towards o-, m- and p-nitrobenzoic acid, o-, m- and p-hydroxybenzoic acid, o-, m- and p-toluic acid and the optical isomers of dansyl-D,L-leucine and R,S-1,1'-binaphthyl-2,2-dihydrogenphosphate. Especially the effect of organic solvents, such as acetonitrile, methanol, dimethylsulphoxide and others were examined in detail. The resolution varied to some extent with the addition of the organic solvent to the polyacrylamide gel and the running buffer solution. The possible mechanism has also been discussed. In addition, quantitative aspects of the separation of stereoisomers using CGE have been studied, showing that both the resolution and accuracy of the determinations were affected by the ratio of the enantiomers.
Authors:
J M Lin; T Nakagama; H Okazawa; X Z Wu; T Hobo
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  354     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  1996 Feb 
Date Detail:
Created Date:  2004-03-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  451-4     Citation Subset:  -    
Affiliation:
Department of Industrial Chemistry, Faculty of Engineering, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, 192-03, Hachioji, Tokyo, Japan.
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