Document Detail

Separation and detection of rare cells in a microfluidic disk via negative selection.
MedLine Citation:
PMID:  21088774     Owner:  NLM     Status:  In-Data-Review    
Cyto-analysis of rare cells often requires separation and detection with each procedure posing substantial challenges. This paper presents a disk-based microfluidic platform for both procedures via an immunomagnetic negative selection process. The microfluidic platform's unique features include a multistage magnetic gradient to trap labeled cells in double trapping areas, drainage of fluid to substantially shorten detection time, and a bin-like regions to capture target cells to facilitate a seamless enumeration process. Proof-of-concept was conducted using MCF7 as target rare cells (stained with anti-cytokeratin-FITC antibodies) spiked into Jurkat Clone E6-1 non-target cells (labeled with anti-CD45-PE and anti-PE BD magnetic beads). Then, mononuclear cells (MNC) from healthy blood donors were mixed with MCF7s, modeling rare cells, and tested in the disk. Results show a non-linear magnetic coupling effect of the multistage magnet substantially increased the trapping efficacy over that of a single magnet, contributing to the depletion rate of Jurkats, which reaches 99.96%. Detection time is extensively shortened by depletion of 95% of non-cell-containing fluid in the collection area. The average yield of detected MCF7 cells is near-constant 60 ± 10% over a wide range of rarity from 10(-3) to 10(-6) and this yield also holds for the MCF7/MNC complex mixture. Comparison with autoMACS and BD IMagnet separators revealed the average yield from the disk (60%) is superior to that of autoMACS (37.3%) and BD IMagnet (48.3%). The advantages of near-constant yield, roughly 30 min of operation, an acceptable level of cell loss, and potentially low cost system should aid in cyto-analysis of rare cells.
Chen-Lin Chen; Ken-Chao Chen; Yu-Cheng Pan; Tai-Ping Lee; Lo-Chang Hsiung; Cheng-Ming Lin; Chang-Yu Chen; Ching-Hung Lin; Bor-Luen Chiang; Andrew M Wo
Related Documents :
1175504 - Uptake and conversion of progesterone and testosterone to 5alpha-reduced products by en...
2773624 - Familial acromegaly.
8391364 - Application of a percoll density gradient to separate and enrich porcine pituitary cell...
19159354 - Immunohistochemistry of connexin 43 throughout anterior pituitary gland in a transgenic...
24317894 - The lysopinedehydrogenase gene used as a marker for the selection of octopine crown gal...
25217104 - The p53/mouse double minute 2 homolog complex deregulation in merlin-deficient tumours.
Publication Detail:
Type:  Journal Article     Date:  2010-11-18
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  474-83     Citation Subset:  IM    
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Nanoparticle-based mass spectrometry for the analysis of biomolecules.
Next Document:  Iron-mediated one-pot formal nitrocyclization onto unactivated alkenes.