Document Detail


Separation and detection of multiple pathogens in a food matrix by magnetic SERS nanoprobes.
MedLine Citation:
PMID:  21136046     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A rapid and sensitive method was developed here for separation and detection of multiple pathogens in food matrix by magnetic surface-enhanced Raman scattering (SERS) nanoprobes. Silica-coated magnetic probes (MNPs@SiO(2)) of ~100 nm in diameter were first prepared via the reverse microemulsion method using cetyltrimethylammonium bromide as a surfactant and tetraethyl orthosilicate as the silica precursor. The as-prepared MNPs@SiO(2) were functionalized with specific pathogen antibodies to first capture threat agents directly from a food matrix followed by detection using an optical approach enabled by SERS. In this scheme, pathogens were first immuno-magnetically captured with MNPs@SiO(2), and pathogen-specific SERS probes (gold nanoparticles integrated with a Raman reporter) were functionalized with corresponding antibodies to allow the formation of a sandwich assay to complete the sensor module for the detection of multiple pathogens in selected food matrices, just changing the kinds of Raman reporters on SERS probes. Here, up to two key pathogens, Salmonella enterica serovar Typhimurium and Staphylococcus aureus, were selected as a model to illustrate the probability of this scheme for multiple pathogens detection. The lowest cell concentration detected in spinach solution was 10(3) CFU/mL. A blind test conducted in peanut butter validated the limit of detection as 10(3) CFU/mL with high specificity, demonstrating the potential of this approach in complex matrices.
Authors:
Yuling Wang; Sandeep Ravindranath; Joseph Irudayaraj
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-12-07
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  399     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1271-8     Citation Subset:  IM    
Affiliation:
Physiological Sensing Facility, Bindley Biosciences Center, Agricultural and Biological Engineering, Purdue University, 225 S. University Street, West Lafayette, IN 47907, USA.
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