Document Detail


Separation of creatine-kinase isoenzymes on ion-exchange mini-columns for diagnostic purposes.
MedLine Citation:
PMID:  7336099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For diagnostic purposes, creatine-kinase (CK) isoenzymes were separated on mini-columns of DEAE Sephadex A50 from the serum of 55 patients with myocardial infarction or cerebral disturbances and from 45 normal subjects. The optimum conditions of separation were determined by separation of CK isoenzymes (CK--MM, CK--MB, CK--BB) from human organ homogenates (skeletal muscle, myocardium, brain). In the patients with myocardial infarction the CK-MB isoenzyme activity amounted to more than 6% of the total enzyme value. The chromatogram in these cases was similar to that of human myocardium homogenate. The CK-BB isoenzyme was found only in patients with severe cerebral disturbances (cerebral hemorrhage). In such cases the chromatogram was similar to that of human brain homogenate. Therefore, by associating the assay of serum CK total activity with the separation of CK isoenzymes it was proved that the CK--MB and CK--BB isoenzymes can supply a useful test in differential diagnosis.
Authors:
Z Chirulescu; C Chiriloiu; A Suciu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Médecine interne     Volume:  19     ISSN:  0377-1202     ISO Abbreviation:  Med Interne     Publication Date:    1981 Oct-Dec
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7506353     Medline TA:  Med Interne     Country:  ROMANIA    
Other Details:
Languages:  eng     Pagination:  383-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Brain / enzymology
Brain Diseases / diagnosis*,  enzymology
Chromatography, Ion Exchange
Creatine Kinase / analysis*,  blood
Humans
Isoenzymes
Myocardial Infarction / diagnosis*,  enzymology
Myocardium / enzymology
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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