| Separation of creatine-kinase isoenzymes on ion-exchange mini-columns for diagnostic purposes. | |
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MedLine Citation:
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PMID: 7336099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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For diagnostic purposes, creatine-kinase (CK) isoenzymes were separated on mini-columns of DEAE Sephadex A50 from the serum of 55 patients with myocardial infarction or cerebral disturbances and from 45 normal subjects. The optimum conditions of separation were determined by separation of CK isoenzymes (CK--MM, CK--MB, CK--BB) from human organ homogenates (skeletal muscle, myocardium, brain). In the patients with myocardial infarction the CK-MB isoenzyme activity amounted to more than 6% of the total enzyme value. The chromatogram in these cases was similar to that of human myocardium homogenate. The CK-BB isoenzyme was found only in patients with severe cerebral disturbances (cerebral hemorrhage). In such cases the chromatogram was similar to that of human brain homogenate. Therefore, by associating the assay of serum CK total activity with the separation of CK isoenzymes it was proved that the CK--MB and CK--BB isoenzymes can supply a useful test in differential diagnosis. |
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Authors:
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Z Chirulescu; C Chiriloiu; A Suciu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Médecine interne Volume: 19 ISSN: 0377-1202 ISO Abbreviation: Med Interne Publication Date: 1981 Oct-Dec |
Date Detail:
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Created Date: 1982-05-21 Completed Date: 1982-05-21 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7506353 Medline TA: Med Interne Country: ROMANIA |
Other Details:
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Languages: eng Pagination: 383-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Brain
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enzymology Brain Diseases / diagnosis*, enzymology Chromatography, Ion Exchange Creatine Kinase / analysis*, blood Humans Isoenzymes Myocardial Infarction / diagnosis*, enzymology Myocardium / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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