Document Detail


Separation of complex mixtures of fluorobenzoic acids by capillary electrophoresis.
MedLine Citation:
PMID:  19142912     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorobenzoic acids are important intermediates in the synthesis of antibacterial drugs. Conditions for the separation of mixtures of twenty-five acids by CE have been optimized. A set of conditions with phosphate buffer (pH 3.1), successive multiple ionic-polymer layer (SMIL) coating capillary, and negative separation voltage provided a short time separation based on the difference in the acidic dissociation constant (pK(a)) of the sample acids. Addition of ACN to the separation solution improved the selectivity resulting in a broader distribution of migration times of the samples. The addition of tetradecyltrimethylammonium chloride below the CMC was also effective in changing the migration pattern of fluorobenzoic acids. Furthermore, practical utility was demonstrated through quantitative studies on the optimized condition including the durability of the SMIL coating.
Authors:
Tsuguhide Isemura; Fumihiko Kitagawa; Koji Otsuka
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of separation science     Volume:  32     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-04-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  381-7     Citation Subset:  IM    
Affiliation:
Analytical Science & TechnologyFunction, Asahi Glass Co., Ltd.Research Center, Yokohama,Kanagawa, Japan. tsuguhide-isemura@agc.co.jp
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MeSH Terms
Descriptor/Qualifier:
Benzoic Acids / chemistry,  isolation & purification*
Chromatography, High Pressure Liquid
Cyclodextrins / chemistry
Electrophoresis, Capillary / methods*
Fluorine Compounds / chemistry,  isolation & purification*
Hydrogen-Ion Concentration
Molecular Structure
Silicon Dioxide / chemistry
Surface-Active Agents / chemistry
Chemical
Reg. No./Substance:
0/Benzoic Acids; 0/Cyclodextrins; 0/Fluorine Compounds; 0/Surface-Active Agents; 7631-86-9/Silicon Dioxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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