| Separation of clavulanic acid from fermented broth of amino acids by an aqueous two-phase system and ion-exchange adsorption. | |
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MedLine Citation:
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PMID: 21683811 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The clavulanic acid is a substance which inhibits the β-lactamases used with penicillins for therapeutic treatment. After the fermentation, by-products of low molecular weight such as amino acids lysine, histidine, proline and tyrosine are present in the fermented broth. To remove these impurities the techniques of extraction by an aqueous two-phase system of 17% polyethylene glycol molecular weight 600 and 15% potassium phosphate were used for a partial purification. A subsequent ion-exchange adsorption was used for the recuperation of the clavulanic acid of the top phase and purification getting a concentration factor of 2 and purification of 100% in relation to the amino acids lysine, histidine, proline and tyrosine. |
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Authors:
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Clovis Sacardo da Silva; Maressa Fabiano Cuel; Verônica Orlandin Barreto; Wu Hong Kwong; Carlos O Hokka; M Barboza |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-12 |
Journal Detail:
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Title: New biotechnology Volume: - ISSN: 1876-4347 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101465345 Medline TA: N Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier B.V. |
Affiliation:
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Department of Chemical Engineering, Universidade Federal de São Carlos, Cx. Postal 676, CEP 13565-905, São Carlos, SP, Brazil; Department of Chemical Engineering, Universidade Federal do Triângulo Mineiro, Instituto de Ciências Tecnológicas e Exatas, Avenida Dr. Randolfo Borges Jr. 1250, Univerdicidade-Uberaba, MG, Brazil. |
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