Document Detail


Separation of chlorogenic acid and concentration of trace caffeic acid from natural products by pH-zone-refining countercurrent chromatography.
MedLine Citation:
PMID:  23625646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chlorogenic acid and caffeic acid were selected as test samples for separation by the pH-zone-refining countercurrent chromatography (CCC). The separation of these test samples was performed with a two-phase solvent system composed of methyl-tert-butyl-ether/acetonitrile/water at a volume ratio of 4:1:5 v/v/v where trifluoroacetic acid (TFA; 8 mM) was added to the organic stationary phase as a retainer and NH4 OH (10 mM) to the aqueous mobile phase as an eluter. Chlorogenic acid was successfully separated from Flaveria bidentis (L.) Kuntze (F. bidentis) and Lonicerae Flos by pH-zone-refining CCC, a slightly polar two-phase solvent system composed of methyl-tert-butyl-ether/acetonitrile/n-butanol/water at a volume ratio of 4:1:1:5 v/v/v/v was selected where TFA (3 mM) was added to the organic stationary phase as a retainer and NH4 OH (3 mM) to the aqueous mobile phase as an eluter. A 16.2 mg amount of chlorogenic acid with the purity of 92% from 1.4 g of F. bidentis, and 134 mg of chlorogenic acid at the purity of 99% from 1.3 g of crude extract of Lonicerae Flos have been obtained. These results suggest that pH-zone-refining CCC is suitable for the isolation of the chlorogenic acid from the crude extracts of F. bidentis and Lonicerae Flos.
Authors:
Yuanyuan Lu; Genlai Dong; Yanxiang Gu; Yoichiro Ito; Yun Wei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-05-27
Journal Detail:
Title:  Journal of separation science     Volume:  36     ISSN:  1615-9314     ISO Abbreviation:  J Sep Sci     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-02     Completed Date:  2014-01-21     Revised Date:  2014-07-02    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2210-5     Citation Subset:  IM    
Copyright Information:
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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MeSH Terms
Descriptor/Qualifier:
Biological Products / chemistry*
Caffeic Acids / isolation & purification*
Chlorogenic Acid / isolation & purification*
Countercurrent Distribution / methods*
Flaveria / chemistry
Hydrogen-Ion Concentration*
Lonicera / chemistry
Grant Support
ID/Acronym/Agency:
Z99 HL999999/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Products; 0/Caffeic Acids; 318ADP12RI/Chlorogenic Acid; U2S3A33KVM/caffeic acid
Comments/Corrections

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