| Separation and characterization of two different species of rat angiotensinogen. | |
| | |
MedLine Citation:
|
PMID: 6816288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Two distinct species of rat angiotensinogen (A-1 and A-2) were purified from plasma of nephrectomized rats by combining ammonium sulfate fractionation, chromatography on Blue Sepharose and SP-Sephadex, gel filtration and preparative polyacrylamide gel electrophoresis. Separation of the two species was accomplished in the SP-Sephadex chromatography step, A-1 eluting before A-2. The two angiotensinogen species had identical electrophoretic mobilities on analytical polyacrylamide gel electrophoresis, but differed in their apparent molecular weights as obtained by SDS-gel electrophoresis (A-1, Mr 60 000; A-2, Mr 56 400). In analytical isoelectric focusing each species displayed a characteristic double band with isoelectric points of 4.54 and 4.60 for A-1, and 4.69 and 4.76 for A-2. These physicochemical differences can be accounted for by the difference in carbohydrate content: A-1, when compared to A-2, had a higher content of sialic acid (5.0 and 2.1 mol/mol), neutral hexoses (10.2 and 5.9 mol/mol) and aminohexoses (10.5 and 7.0 mol/mol, respectively). Antiserum raised against rat angiotensinogen crossreacted completely with both angiotensinogens. Both species could also be isolated from plasma of non-nephrectomized rats, which indicates that they may be present under physiological conditions. The physiological significance of the occurrence of these species of angiotensinogen is still unknown. |
| | |
Authors:
|
U Hilgenfeldt; E Hackenthal |
Related Documents
:
|
1779438 - The in vitro and in vivo cytotoxicity of menadione (vitamin k3) against rat transplanta... 7037168 - Colchicine affects the distribution of isotope-labeled h2o and extracellular markers in... 22358528 - Assay systems for screening food components that have anti-proliferative and anti-invas... 16218878 - Degradation of the sodium taurocholate cotransporting polypeptide (ntcp) by the ubiquit... 14714098 - Oxys rats as a model of senile cataract. 8016408 - Evidence for the presence of prolyl oligopeptidase and its endogenous inhibitor in neon... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 708 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1982 Nov |
Date Detail:
|
Created Date: 1983-02-14 Completed Date: 1983-02-14 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 335-42 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiotensinogen
/
blood,
isolation & purification* Angiotensins / isolation & purification* Animals Immunodiffusion Kinetics Male Molecular Weight Nephrectomy Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Angiotensins; 11002-13-4/Angiotensinogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Specificities of antisera directed against soybean lipoxygenases-1 and -2 and purification of lipoxy...
Next Document: Comparative studies on aldose reductase from bovine, rat and human lens.