Document Detail

Separation and characterization of antioxidants from Spirulina platensis microalga combining pressurized liquid extraction, TLC, and HPLC-DAD.
MedLine Citation:
PMID:  16318207     Owner:  NLM     Status:  MEDLINE    
A new procedure has been developed to separate and characterize antioxidant compounds from Spirulina platensis microalga based on the combination of pressurized liquid extraction (PLE) and different chromatographic procedures, such as TLC, at preparative scale, and HPLC with a diode array detector (DAD). Different solvents were tested for PLE extraction of antioxidants from S. platensis microalga. An optimized PLE process using ethanol (generally recognized as safe, GRAS) as extraction solvent has been obtained that provides natural extracts with high yields and good antioxidant properties. TLC analysis of this ethanolic extract obtained at 115 degrees C for 15 min was carried out and the silica layer was stained with a DPPH (diphenyl-pycril-hydrazyl) radical solution to determine the antioxidant activity of different chromatographic bands. Next, these colored bands were collected for their subsequent analysis by HPLC-DAD, revealing that the compounds with the most important antioxidant activity present in Spirulina extracts were carotenoids, as well as phenolic compounds and degradation products of chlorophylls.
Laura Jaime; Jos? A Mendiola; Miguel Herrero; Cristina Soler-Rivas; Susana Santoyo; F Javier Se?orans; Alejandro Cifuentes; Elena Ib??ez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  28     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-12-01     Completed Date:  2008-02-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2111-9     Citation Subset:  IM    
Secci?n Departamental Ciencias de la Alimentaci?n, Universidad Aut?noma de Madrid, Ciudad Universitaria de Cantoblanco, Madrid, Spain.
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MeSH Terms
Antioxidants / analysis*,  chemistry
Chromatography, High Pressure Liquid*
Chromatography, Thin Layer*
Cyanobacteria / chemistry*
Reg. No./Substance:

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