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Separation and characterization of acetyl and non-acetyl hemicelluloses of Arundo donax by ammonium sulphate precipitation.
MedLine Citation:
PMID:  22480383     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Delignified Arundo donaxl was sequentially extracted with DMSO, saturated barium hydroxide and 1.0 M aqueous NaOH solution. The yields of the soluble fractions were 10.2, 6.7 and 10.0% (w/w), respectively, of the dry Arundo donaxl materials. The DMSO-, Ba(OH)2- and NaOH-soluble hemicellulosic fractions were further fractionated into two subfractions by gradient 50% and 80% saturation ammonium sulphate precipitation, respectively. Monosaccharide, molecular weight, FT-IR, 1D (1H and 13C) and 2D (HSQC) NMR analysis revealed the differences in structural characteristics and physicochemical properties among the subfractions. The subfractions precipitated with 50% saturation ammonium sulphate had lower arabinose/xylose and glucuronic acid/xylose ratios, but had higher molecular weight than those of the subfractions precipitated by 80% saturation ammonium sulphate. FT-IR and NMR analysis revealed that the highly acetylated DMSO-soluble hemicellulosic subfraction (HD50) could be precipitated with a relatively lower concentration of 50% saturated ammonium sulphate, and thus the gradient ammonium sulphate precipitation technique could discriminate acetyl and non-acetyl hemicelluloses. It was found that the DMSO-soluble subfraction HD50 precipitated by 50% saturated ammonium sulphate mainly consisted of lowly substituted O-acetyl arabino-4-O-methylglucurono xylan with terminal units of arabinose linked on position 3 of xylose, 4-O-methylglucuronic acid residues linked on position 2 of the xylan bone, and the acetyl groups (degree of acetylation, 37%) linked on position 2 or 3. The DMSO-soluble subfraction HD80 precipitated by 80% saturated ammonium sulphate was mainly composed of highly substituted arabino-4-O-methylglucurono xylan and β-D-glucan.
Authors:
Feng Peng; Jing Biang; Pai Peng; Huan Xiao; Jun-Li Ren; Feng Xu; Run-Cang Sun
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-5
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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