| Separation and characterization of acetyl and non-acetyl hemicelluloses of Arundo donax by ammonium sulphate precipitation. | |
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MedLine Citation:
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PMID: 22480383 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Delignified Arundo donaxl was sequentially extracted with DMSO, saturated barium hydroxide and 1.0 M aqueous NaOH solution. The yields of the soluble fractions were 10.2, 6.7 and 10.0% (w/w), respectively, of the dry Arundo donaxl materials. The DMSO-, Ba(OH)2- and NaOH-soluble hemicellulosic fractions were further fractionated into two subfractions by gradient 50% and 80% saturation ammonium sulphate precipitation, respectively. Monosaccharide, molecular weight, FT-IR, 1D (1H and 13C) and 2D (HSQC) NMR analysis revealed the differences in structural characteristics and physicochemical properties among the subfractions. The subfractions precipitated with 50% saturation ammonium sulphate had lower arabinose/xylose and glucuronic acid/xylose ratios, but had higher molecular weight than those of the subfractions precipitated by 80% saturation ammonium sulphate. FT-IR and NMR analysis revealed that the highly acetylated DMSO-soluble hemicellulosic subfraction (HD50) could be precipitated with a relatively lower concentration of 50% saturated ammonium sulphate, and thus the gradient ammonium sulphate precipitation technique could discriminate acetyl and non-acetyl hemicelluloses. It was found that the DMSO-soluble subfraction HD50 precipitated by 50% saturated ammonium sulphate mainly consisted of lowly substituted O-acetyl arabino-4-O-methylglucurono xylan with terminal units of arabinose linked on position 3 of xylose, 4-O-methylglucuronic acid residues linked on position 2 of the xylan bone, and the acetyl groups (degree of acetylation, 37%) linked on position 2 or 3. The DMSO-soluble subfraction HD80 precipitated by 80% saturated ammonium sulphate was mainly composed of highly substituted arabino-4-O-methylglucurono xylan and β-D-glucan. |
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Authors:
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Feng Peng; Jing Biang; Pai Peng; Huan Xiao; Jun-Li Ren; Feng Xu; Run-Cang Sun |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-5 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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