| Separation of amino acid phenylthiohydantoin derivatives by high-pressure liquid chromatography. | |
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MedLine Citation:
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PMID: 3728965 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Separation of the phenylthiohydantoin (PTH) derivatives of all 20 common amino acids is accomplished in approximately 11 min with excellent resolution by using high-pressure liquid chromatography. The chromatography is achieved at 50 degrees C on an Altex reversed-phase PTH-C18 column in an ammonium acetate-buffered acetonitrile, pH 4.5, mobile phase. Simple isocratic and linear gradient steps are used. Retention times for the various PTH-amino acids are very reproducible. Because the baseline is flat and free of background noise, PTH-amino acids can be detected in the low picomole range. The simplicity of this chromatographic system allows it to be easily automated. |
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Authors:
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J L Meuth; J L Fox |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 154 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-07-25 Completed Date: 1986-07-25 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 478-84 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / isolation & purification* Chromatography, High Pressure Liquid Hydantoins / isolation & purification* Hydrogen-Ion Concentration Microchemistry Phenylthiohydantoin / analogs & derivatives, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Hydantoins; 2010-15-3/Phenylthiohydantoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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