Document Detail


Separation of active and inactive fractions from starved culture of Vibrio parahaemolyticus by density dependent cell sorting.
MedLine Citation:
PMID:  16329866     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The co-existence of physiologically different cells in bacterial cultures is a general phenomenon. We have examined the applicability of the density dependent cell sorting (DDCS) method to separate subpopulations from a long-term starvation culture of Vibrio parahaemolyticus. The cells were subjected to Percoll density gradient and separated into 12 fractions of different buoyant densities, followed by measuring the cell numbers, culturability, respiratory activity and leucine incorporation activity. While more than 78% of cells were in lighter fractions, about 95% of culturable cells were present in heavier fractions. The high-density subpopulations also had high proportion of cells capable of forming formazan granules. Although this was accompanied by the cell specific INT-reduction rate, both leucine incorporation rates and INT-reduction rates per cell had a peak at mid-density fraction. The present results indicated that DDCS could be used to separate subpopulations of different physiological conditions.
Authors:
Binaya Bhusan Nayak; Eriko Kamiya; Tomohiko Nishino; Minoru Wada; Masahiko Nishimura; Kazuhiro Kogure
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  51     ISSN:  0168-6496     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-12-06     Completed Date:  2005-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  179-86     Citation Subset:  IM    
Affiliation:
Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Tokyo 164 8639, Japan. binayan@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Cell Respiration
Cell Separation / methods*
Centrifugation, Density Gradient / methods*
Vibrio parahaemolyticus / cytology,  isolation & purification*,  metabolism*

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