Document Detail


Separation of cis-trans phospholipid isomers using reversed phase LC with high resolution MS detection.
MedLine Citation:
PMID:  22656324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The increased presence of synthetic trans fatty acids into western diets has been shown to have deleterious effects on physiology and raising an individual's risk of developing metabolic disease, cardiovascular disease, and stroke. The importance of these fatty acids for health and the diversity of their (patho) physiological effects suggest that not only should the free trans fatty acids be studied but also monitoring the presence of these fats into the side chains of biological lipids, such as glycerophospholipids, is also essential. We developed a high resolution LC-MS method that quantitatively monitors the major lipid classes found in biospecimens in an efficient, sensitive, and robust manner while also characterizing individual lipid side chains through the use of high energy collisional dissociation (HCD) fragmentation and chromatographic alignment. We herein show how this previously described reversed phase method can baseline separate the cis-trans isomers of phosphatidylglycerol and phosphatidylcholine (PC) with two 18:1 side chains, in both positive and negative mode, as neat solutions and when spiked into a biological matrix. Endogenous PC (18:1/18:1)-cis and PC (18:1/18:1)-trans isomers were examined in mitochondrial and serum profiling studies, where rats were fed diets enriched in either trans 18:1 fatty acids or cis 18:1 fatty acids. In this study, we determined the cis:trans isomer ratios of PC (18:1/18:1) and related this ratio to dietary composition. This generalized LC-MS method enables the monitoring of trans fats in biological lipids in the context of a nontargeted method, allowing for relative quantitation and enhanced identification of unknown lipids in complex matrixes.
Authors:
Susan S Bird; Vasant R Marur; Irina G Stavrovskaya; Bruce S Kristal
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-12
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-05     Completed Date:  2012-10-30     Revised Date:  2013-07-08    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5509-17     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Brigham and Women's Hospital, Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, United States.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, Reverse-Phase* / methods
Diet
Free Radicals / chemistry
Isomerism
Male
Mass Spectrometry / methods
Mitochondria / chemistry
Phosphatidylcholines / blood,  chemistry,  isolation & purification*
Phosphatidylglycerols / chemistry,  isolation & purification*
Rats
Rats, Inbred F344
Grant Support
ID/Acronym/Agency:
U01 ES016048/ES/NIEHS NIH HHS; U01-ES16048/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Free Radicals; 0/Phosphatidylcholines; 0/Phosphatidylglycerols
Comments/Corrections

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