Document Detail


Separation of B and T lymphocytes by cellular adsorption chromatography with polyamine graft copolymers as column matrices. II. Recovery of adsorbed B cell enriched populations from the column.
MedLine Citation:
PMID:  2804229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymers were developed for column adsorbents for separating lymphocyte subpopulations collected from rat mesenteric lymph nodes. Bead-shaped adsorbents were prepared by coating poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer on glass beads. Separation features of the column packed with poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer-coated glass beads were evaluated under various operating conditions. Separation efficacy, AB/AT, was significantly affected by the infusion rate of lymphocyte suspension into the column, increasing with decreasing infusion rate. T cell purity in the column effluent was almost 95% at the infusion rate of 0.1 ml/min. The infusion rate was also found to affect the detachment of adsorbed lymphocytes from the poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer surface by mechanical pipetting; lymphocytes adsorbed at a low infusion rate were able to be detached quantitatively by suspending the lymphocyte-adsorbed beads with gentle pipetting. The detached lymphocytes thus obtained were confirmed to be enriched in B cells 1.4 times the initial mixture of B and T cells. The addition of albumin in the medium was found to affect the processes of adsorption and recovery of lymphocyte.
Authors:
A Maruyama; T Tsuruta; K Kataoka; Y Sakurai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  10     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-12-21     Completed Date:  1989-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  393-9     Citation Subset:  IM    
Affiliation:
Department of Industrial Chemistry, Faculty of Engineering, Science University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Animals
B-Lymphocytes / drug effects*
Cell Aggregation / drug effects
Cell Separation
Chromatography, Affinity
Microspheres*
Polyamines / pharmacology*
Polyhydroxyethyl Methacrylate / pharmacology*
Polymethacrylic Acids / pharmacology*
Rats
Rats, Inbred Strains
T-Lymphocytes / drug effects
Chemical
Reg. No./Substance:
0/Polyamines; 0/Polymethacrylic Acids; 0/poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; 25249-16-5/Polyhydroxyethyl Methacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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