| Separation of B and T lymphocytes by cellular adsorption chromatography with polyamine graft copolymers as column matrices. II. Recovery of adsorbed B cell enriched populations from the column. | |
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MedLine Citation:
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PMID: 2804229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymers were developed for column adsorbents for separating lymphocyte subpopulations collected from rat mesenteric lymph nodes. Bead-shaped adsorbents were prepared by coating poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer on glass beads. Separation features of the column packed with poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer-coated glass beads were evaluated under various operating conditions. Separation efficacy, AB/AT, was significantly affected by the infusion rate of lymphocyte suspension into the column, increasing with decreasing infusion rate. T cell purity in the column effluent was almost 95% at the infusion rate of 0.1 ml/min. The infusion rate was also found to affect the detachment of adsorbed lymphocytes from the poly(2-hydroxyethyl methacrylate)-graft-polyamine copolymer surface by mechanical pipetting; lymphocytes adsorbed at a low infusion rate were able to be detached quantitatively by suspending the lymphocyte-adsorbed beads with gentle pipetting. The detached lymphocytes thus obtained were confirmed to be enriched in B cells 1.4 times the initial mixture of B and T cells. The addition of albumin in the medium was found to affect the processes of adsorption and recovery of lymphocyte. |
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Authors:
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A Maruyama; T Tsuruta; K Kataoka; Y Sakurai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomaterials Volume: 10 ISSN: 0142-9612 ISO Abbreviation: Biomaterials Publication Date: 1989 Aug |
Date Detail:
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Created Date: 1989-12-21 Completed Date: 1989-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 393-9 Citation Subset: IM |
Affiliation:
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Department of Industrial Chemistry, Faculty of Engineering, Science University of Tokyo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals B-Lymphocytes / drug effects* Cell Aggregation / drug effects Cell Separation Chromatography, Affinity Microspheres* Polyamines / pharmacology* Polyhydroxyethyl Methacrylate / pharmacology* Polymethacrylic Acids / pharmacology* Rats Rats, Inbred Strains T-Lymphocytes / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Polyamines; 0/Polymethacrylic Acids; 0/poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; 25249-16-5/Polyhydroxyethyl Methacrylate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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