Document Detail

Separation of oligo/polymers of 5-N-acetylneuraminic acid, 5-N-glycolylneuraminic acid, and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid by high-performance anion-exchange chromatography with pulsed amperometric detector.
MedLine Citation:
PMID:  9245445     Owner:  NLM     Status:  MEDLINE    
A sensitive and efficient method to analyze oligo/ poly-sialic acids containing alpha2-8-linked 5-N-acetylneuraminic acid (Neu5Ac), 5-N-glycolylneuraminic acid (Neu5Gc), and deaminated neuraminic acid (KDN) using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD-2) has been developed. Using a CarboPac PA-100 column and sodium nitrate as the pushing agent, polymers in colominic acid with degree of polymerization (DP) up to 80 were separated in 68 min. A similar DP-based resolution was also obtained on a CarboPac PA-1 column. The elution ladders of the Neu5Ac, Neu5Gc, and KDN series were sufficiently different to be used as diagnostic indices. This technique was applied to identification of the sialic acid components in a polysialoglycoprotein (PSGP) sample as well as monitoring the oligo/poly-KDN-containing fractions during the purification of KDN-containing glycoprotein (KDN-gp). The maximum DPs of oligo-Neu5Gc and oligo-KDN that can be detected in PSGP and KDN-gp hydrolysates were 11 and 8, respectively. The high sensitivity of this method was demonstrated by the quantification of Neu5Ac oligomers. Distributions of the monomer and oligo/polymers in the acid and enzymatic hydrolysates of colominic acid and PSGP under different conditions were also studied.
Y Zhang; Y Inoue; S Inoue; Y C Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  250     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1997 Aug 
Date Detail:
Created Date:  1997-09-24     Completed Date:  1997-09-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  245-51     Citation Subset:  IM    
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
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MeSH Terms
Anion Exchange Resins
N-Acetylneuraminic Acid / chemistry,  isolation & purification*
Neuraminic Acids / chemistry,  isolation & purification*
Sialic Acids / chemistry,  isolation & purification
Sugar Acids / chemistry,  isolation & purification*
Grant Support
Reg. No./Substance:
0/Anion Exchange Resins; 0/Neuraminic Acids; 0/Sialic Acids; 0/Sugar Acids; 1113-83-3/N-glycolylneuraminic acid; 131-48-6/N-Acetylneuraminic Acid; 22594-61-2/3-deoxyglycero-galacto-nonulosonic acid
Erratum In:
Anal Biochem 1997 Oct 15;252(2):326

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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