Document Detail

Separate control of regulatory volume increase and Na+-H+ exchange by cultured renal cells.
MedLine Citation:
PMID:  2839037     Owner:  NLM     Status:  MEDLINE    
Suspensions of OK cells (a continuous epithelioid cell line from opossum kidney) are examined by electronic cell sizing, measurements of intracellular pH, and measurements of cellular Na+ and K+. The response of the cells to hypertonic solutions is evaluated in most detail. When shrunken by exposure to hyperosmotic medium (430 mosmol/kg), the cells do not demonstrate a regulatory volume increase (RVI) independent of the solute that is used to increase osmolality [NaCl, N-methyl-D-glucamine-HCl (NMGCl), or sucrose]. In contrast, when cells are preexposed to 190 mosmol/kg medium and then shrunken by exposure to 310 mosmol/kg medium, a volume increase is observed after the addition of 120 mosmol/kg NaCl or NMGCl, but not sucrose. This RVI is sensitive to 1 mM furosemide and removal of Na+ or K+ from the medium, but it is not inhibited by 1 mM amiloride. In the presence of a propionate-induced cellular acidification, a Na+-H+ exchanger in the cells is shown to have a large capacity for net solute uptake and to be inhibited by 1 mM amiloride. Net solute uptake by the Na+-H+ exchanger is sensitive to addition of parathyroid hormone or 8-bromoadenosine 3',5'-cyclic monophosphate but is not stimulated in response to cell shrinkage.
M H Montrose; C Knoblauch; H Murer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  255     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-11     Completed Date:  1988-08-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C76-85     Citation Subset:  IM    
Institute of Physiology, University of Zurich, Switzerland.
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Amiloride / pharmacology
Carrier Proteins / metabolism*
Cells, Cultured
Furosemide / pharmacology
Hydrogen-Ion Concentration
Kidney / metabolism*
Meglumine / pharmacology
Osmolar Concentration
Parathyroid Hormone / pharmacology
Propionic Acids / pharmacology
Sodium-Hydrogen Antiporter
Reg. No./Substance:
0/Carrier Proteins; 0/Parathyroid Hormone; 0/Propionic Acids; 0/Sodium-Hydrogen Antiporter; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 2609-46-3/Amiloride; 54-31-9/Furosemide; 6284-40-8/Meglumine; 79-09-4/propionic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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