| Sentinel lymph node mapping using near-infrared fluorescent methylene blue. | |
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MedLine Citation:
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PMID: 19332308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methylene blue (MB) is a safe, low cost, and common blue dye remaining a popular choice for sentinel lymph node (SLN) mapping. However, the blue-dyed SLN in deep animal tissue could be seen only after a surgical management. Here the fluorescence properties of MB were investigated using a fluorescence spectrometer and a wavelength-resolved fluorescence spectral in vivo imaging system, and MB has been demonstrated for near-infrared (NIR) fluorescence mapping of SLN. When MB was injected intradermally into the second row breast of a rabbit, the lymphatic flow and axillary SLN could be observed directly through the NIR fluorescence emitted from the MB trapped in the deep tissue, which would eliminate the need for surgical management, and this fluorescence without any radioactivity was retained in the SLN for hours. The node also could be identified synchronously by the blue color and fluorescence of the MB after a surgical management. Using MB NIR fluorescence for SLN mapping may have great advantages over the traditional method. |
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Authors:
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Maoquan Chu; Yinghan Wan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bioscience and bioengineering Volume: 107 ISSN: 1347-4421 ISO Abbreviation: J. Biosci. Bioeng. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-31 Completed Date: 2009-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888800 Medline TA: J Biosci Bioeng Country: Japan |
Other Details:
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Languages: eng Pagination: 455-9 Citation Subset: IM |
Affiliation:
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School of Life Science and Technology, Tongji University, Shanghai, China. mqchu98@tongji.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chloral Hydrate / pharmacology Fluorescence Humans Indicators and Reagents Lymph Nodes / cytology, pathology*, physiology* Mammary Glands, Animal / cytology Methylene Blue* Neoplasms / pathology Rabbits Sentinel Lymph Node Biopsy / methods* Skin / cytology Spectrometry, Fluorescence / methods Spectrophotometry / methods Swine |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 302-17-0/Chloral Hydrate; 61-73-4/Methylene Blue |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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