| Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations. | |
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MedLine Citation:
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PMID: 20135117 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fed-batch cultivations of Streptomyces coelicolor, producing the antibiotic actinorhodin, were monitored online by multiwavelength fluorescence spectroscopy and off-gas analysis. Partial least squares (PLS), locally weighted regression, and multilinear PLS (N-PLS) models were built for prediction of biomass and substrate (casamino acids) concentrations, respectively. The effect of combination of fluorescence and gas analyzer data as well as of different variable selection methods was investigated. Improved prediction models were obtained by combination of data from the two sensors and by variable selection using a genetic algorithm, interval PLS, and the principal variables method, respectively. A stepwise variable elimination method was applied to the three-way fluorescence data, resulting in simpler and more accurate N-PLS models. The prediction models were validated using leave-one-batch-out cross-validation, and the best models had root mean square error of cross-validation values of 1.02 g l(-1) biomass and 0.8 g l(-1) total amino acids, respectively. The fluorescence data were also explored by parallel factor analysis. The analysis revealed four spectral profiles present in the fluorescence data, three of which were identified as pyridoxine, NAD(P)H, and flavin nucleotides, respectively. |
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Authors:
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Peter Odman; Claus Lindvald Johansen; Lisbeth Olsson; Krist V Gernaey; Anna Eliasson Lantz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-05 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 86 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-22 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 1745-59 Citation Subset: IM |
Affiliation:
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Department of Systems Biology, Technical University of Denmark, Building 223, DK-2800, Kgs Lyngby, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms Amino Acids / metabolism Anthraquinones / metabolism Biomass Bioreactors* Culture Media Factor Analysis, Statistical Fluorescence Glucose / metabolism Least-Squares Analysis Regression Analysis Spectrometry, Fluorescence Streptomyces coelicolor / growth & development*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Anthraquinones; 0/Culture Media; 1397-77-9/actinorhodin; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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