Document Detail


Sensitizing capacity of three methyl alkanesulphonates: a murine in vivo and in vitro model of allergic contact dermatitis.
MedLine Citation:
PMID:  1964034     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A murine model for in vivo and in vitro studies of contact sensitization to methyl alkanesulphonate derivatives has been developed. Contact sensitivity was quantified in vivo by measuring the ear thickness increase, and the influence of the alkyl chain length (hexyl, dodecyl, hexadecyl) was investigated. Long chain methyl alkanesulphonates (dodecyl and hexadecyl) are strong sensitizers, while the shorter alkyl chain, methyl hexanesulphonate, is a weak sensitizer. In vitro lymphocyte blastogenesis was measured by the 3H-thymidine uptake and exhibited a stimulation index between 2 and 8. The results nicely parallelled the in vivo sensitization measurements, except for methyl dodecanesulphonate. This could be explained by the cytotoxic activity of this compound, the most toxic of the three methyl alkanesulphonates tested. Thus, murine sensitization to methyl alkanesulphonates provides a good model system for preliminary investigations of delayed type hypersensitivity mechanisms.
Authors:
R Fraginals; J P Lepoittevin; C Benezra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of dermatological research     Volume:  282     ISSN:  0340-3696     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  1990  
Date Detail:
Created Date:  1991-04-22     Completed Date:  1991-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  455-8     Citation Subset:  IM    
Affiliation:
Laboratoire de Dermatochimie associé au CNRS, Université Louis Pasteur, CHU, Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Alkanesulfonates / adverse effects,  pharmacology*
Animals
Dermatitis, Contact / pathology*
Disease Models, Animal
Drug Hypersensitivity / pathology*
Epidermis / drug effects,  metabolism,  pathology
Female
Lymphocyte Activation / drug effects
Mice
Mice, Inbred BALB C
Thymidine / metabolism
Tritium / diagnostic use
Chemical
Reg. No./Substance:
0/Alkanesulfonates; 10028-17-8/Tritium; 10307-26-3/methyl n-hexylsulfonate; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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