| Sensitization to blackgram in patients with bronchial asthma and rhinitis: clinical evaluation and characterization of allergens. | |
| | |
MedLine Citation:
|
PMID: 16364164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Legumes are important causative agents of type I hypersensitivity in south Asia and Europe but such studies are lacking in Indian population. The present study investigates blackgram sensitization in asthma and rhinitis patients and identifies immunoglobulin E (IgE)-binding proteins. METHODS: Respiratory allergy patients were evaluated using standard questionnaire and skin prick tests (SPT) with common foods and aeroallergens. Blackgram-specific IgE level was estimated by enzyme-linked immunosorbent assay (ELISA) and sensitization was established by a double-blind, placebo-controlled food challenge (DBPCFC). The cross-reactivity of blackgram with other legumes was studied by immunobiochemical methods. RESULT: Of 816 patients, 35 gave history of blackgram hypersensitivity. From these, 16 patients were SPT positive and 14 showed elevated specific IgE (three times of negative control) to blackgram. DBPCFC established blackgram sensitivity in four of 14 patients. Immunoblotting with individual patient's sera recognized eight most prevalent allergens of 78, 56, 47, 43, 40, 30, 28 and 16 kDa. Roasted blackgram showed six major allergens whereas 47, 43 and 28 kDa proteins retained IgE reactivity upon boiling. Blackgram extract required 14 ng of self protein for 50% ELISA inhibition whereas roasted and boiled blackgram required 16 and 120 ng protein. ELISA and immunoblot inhibition show partial inhibition to blackgram proteins by lentil, limabean and pea. CONCLUSION: Blackgram induces IgE-mediated reactions in 1.7% of asthma and rhinitis patients and contains eight major IgE-binding components, of which six retained IgE reactivity after roasting. Blackgram shares allergenicity with lentil and limabean. |
| | |
Authors:
|
D Kumari; R Kumar; S Sridhara; N Arora; S N Gaur; B P Singh |
Related Documents
:
|
10486364 - Characterisation of immune mediator release during the immediate response to segmental ... 11696054 - Ige binding to soluble and insoluble wheat flour proteins in atopic and non-atopic pati... 16782524 - Food allergies and food intolerances. 1442474 - The elimination diet as a diagnostic tool. 15116754 - Migration of substances from food packaging materials to foods. 1396474 - A new simplified dietary history method for measuring intake of energy and macronutrients. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Allergy Volume: 61 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 2006 Jan |
Date Detail:
|
Created Date: 2005-12-20 Completed Date: 2006-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
|
Languages: eng Pagination: 104-10 Citation Subset: IM |
Affiliation:
|
Allergy and Immunology Section, Institute of Genomics and Integrative Biology, Delhi, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adult Age Distribution Allergens / adverse effects, immunology Asthma / diagnosis, epidemiology, immunology* Child Cohort Studies Enzyme-Linked Immunosorbent Assay Fabaceae / adverse effects, immunology* Female Follow-Up Studies Humans Immunization* Immunoblotting Immunoglobulin E / immunology* India / epidemiology Male Middle Aged Questionnaires Respiratory Function Tests Rhinitis, Allergic, Seasonal / diagnosis, epidemiology, immunology* Risk Assessment Sensitivity and Specificity Severity of Illness Index Sex Distribution Skin Tests |
| Chemical | |
Reg. No./Substance:
|
0/Allergens; 37341-29-0/Immunoglobulin E |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: ALOX5 promoter genotype, asthma severity and LTC production by eosinophils.
Next Document: Changes in airway inflammation following nasal allergic challenge in patients with seasonal rhinitis...