Document Detail

Sensitization of thermotolerant SCK cells to hyperthermia and freezing with reduction of intracellular pH: implications for cryosurgery.
MedLine Citation:
PMID:  12619059     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES: During cryosurgery, cells frozen slowly at the outer part of the ice ball undergo severe dehydration and are subject to solute effects injury, which may be caused in part by protein denaturation. This study was undertaken to determine whether heat shock proteins (HSPs), the molecular chaperones that stabilize proteins against denaturation, have a protective effect on cells during slow freezing. In addition, we aimed to determine whether acidic conditions, similar to those found in many solid tumors, would effect this protection. METHODS: SCK cells were frozen at 5 degrees C/min to -10 degrees C or -20 degrees C before or after induction of thermotolerance, and at neutral or low pH conditions. Lethal damage was determined by clonogenics. RESULTS: Clonogenic survival was decreased by 50% in thermotolerant cells frozen to -10 degrees C after culture in acidic conditions (pH 6.6) compared with non-thermotolerant cells cultured at neutral pH. Induction of thermotolerance alone or low pH alone did not significantly sensitize SCK cells to freezing. All treatment groups were equally susceptible to killing when frozen to -20 degrees C. CONCLUSIONS: Our results show that induction of thermal tolerance does not protect SCK cells against subsequent freezing injury and that a low pH environment actually sensitizes these cells to freeze injury.
Abram H Burgher; David J Swanlund; Robert J Griffin; Chang W Song; John C Bischof; Kenneth P Roberts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of surgical oncology     Volume:  82     ISSN:  0022-4790     ISO Abbreviation:  J Surg Oncol     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-05     Completed Date:  2003-04-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0222643     Medline TA:  J Surg Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  160-9     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Urologic Surgery, University of Minnesota, Minneapolis, Minnesota 55455, USA.
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MeSH Terms
Cell Survival*
Heat-Shock Proteins / analysis
Hydrogen-Ion Concentration
Mammary Neoplasms, Animal / pathology*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins

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