Document Detail


Sensitivity to acetic acid, ability to colonize abiotic surfaces and virulence potential of Listeria monocytogenes EGD-e after incubation on parsley leaves.
MedLine Citation:
PMID:  19778346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate how the survival of Listeria monocytogenes on parsley leaves may affect its ability to sustain process-related harsh conditions and its virulence. METHODS AND RESULTS: Parsley seedlings were spot inoculated with stationary phase cells of L. monocytogenes EGD-e and incubated for 15 days. Each day, bacterial cells were harvested and enumerated, and their ability to survive acetic acid challenge (90 min, pH 4.0), to colonize abiotic surfaces and to grow as biofilms was assessed. After a 3-log decrease over the first 48 h, the population stabilized to about 10(6) CFU g(-1) until the sixth day. After the sixth day, L. monocytogenes was no longer detected, even after specific enrichment. Incubation on parsley leaves affected the ability of L. monocytogenes to survive acetic acid challenge (90 min, pH 4.0) and to adhere to stainless steel although the ability to grow as biofilm was preserved. To further investigate these physiological alterations, the mRNA levels of six target genes (bsh, clpC, groEL, inlA, opuC, prfA) was quantified using reverse transcription qPCR after 5 h of incubation on parsley leaves. A decrease was observed in all but one (bsh) target, including groEL and clpC which are involved in resistance to salt and acid. Moreover, the decrease in the levels of inlA, prfA and opuC transcripts after incubation on parsley suggested a repression of some genes involved in pathogenicity. In vitro assessment of mammalian cell adherence and invasion using Caco-2 cells confirmed the repression of the virulence factor InlA; however, the virulence potential in vivo in the chick embryo model was not affected. CONCLUSION: Listeria monocytogenes did undergo rapid changes to adapt its physiology to the phyllosphere. SIGNIFICANCE AND IMPACT OF THE Study: This study highlights the physiological changes undergone by L. monocytogenes during/after survival on parsley leaves.
Authors:
A Rieu; J Guzzo; P Piveteau
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-07
Journal Detail:
Title:  Journal of applied microbiology     Volume:  108     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-05-04     Completed Date:  2010-07-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  560-70     Citation Subset:  IM    
Affiliation:
Laboratoire de Recherche En Vigne et Vin REVV, Université de Bourgogne, IUVV, Dijon, France.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / pharmacology*
Animals
Bacterial Adhesion
Biofilms
Caco-2 Cells
Chick Embryo
Humans
Listeria monocytogenes / genetics,  growth & development,  pathogenicity*,  physiology*
Microbial Viability
Petroselinum / microbiology*
Plant Leaves / microbiology
Polystyrenes
RNA, Bacterial / analysis
RNA, Messenger / analysis
Stainless Steel
Virulence
Chemical
Reg. No./Substance:
0/Polystyrenes; 0/RNA, Bacterial; 0/RNA, Messenger; 12597-68-1/Stainless Steel; 64-19-7/Acetic Acid

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