| Sensitivity of taurine uptake to oxygen-derived reactive substances in MDR and non-MDR cells. | |
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MedLine Citation:
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PMID: 11665819 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In human KB and LoVo cell lines, high affinity taurine uptake was strongly reduced in both a time and dose-dependent manner by cumene hydroperoxide (CH) and to a lesser extent by hydrogen peroxide (H2O2). Uptake-inhibition was greater in multidrug resistant (MDR) cells than in their non-MDR counterparts. Basal taurine efflux was unaffected by the oxidants. Lipid peroxidation levels closely correlated with the uptake inhibition levels, and were greater in MDR cells than in their non-MDR counterparts. The two oxidants reduced the Vmax and, to a lesser extent, the affinity of the transporter for taurine. They also reduced low affinity taurine uptake and, to a lesser extent, taurine diffusion. The composition of the medium used for cell treatment, especially its pyruvate content, greatly affected the H2O2 effect. H2O2- or CH-induced reduction of the high affinity taurine uptake was unaffected by protein kinase C (PKC) inhibitors and by the calmodulin antagonist W-13, ruling out the involvement of PKC and perhaps of calmodulin kinases in their effect. |
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Authors:
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C Wersinger; I H Lelong-Rebel; G Rebel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Amino acids Volume: 21 ISSN: 0939-4451 ISO Abbreviation: Amino Acids Publication Date: 2001 |
Date Detail:
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Created Date: 2001-10-22 Completed Date: 2002-04-23 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9200312 Medline TA: Amino Acids Country: Austria |
Other Details:
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Languages: eng Pagination: 91-117 Citation Subset: IM |
Affiliation:
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UPR 9003 du CNRS, Institut de Recherche Contre les Cancers de I'Appareil Digestif, Hĵpitaux Universitaires, Strasbourg, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Benzene Derivatives
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pharmacology* Biological Transport / drug effects Buffers Calmodulin / antagonists & inhibitors Drug Resistance, Multiple* Free Radicals / pharmacology Humans Hydrogen Peroxide / pharmacology* KB Cells Lipid Peroxidation Oxidants / pharmacology Oxidative Stress* Protein Kinase C / antagonists & inhibitors Pyruvic Acid / metabolism Reactive Oxygen Species / pharmacology* Taurine / metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Benzene Derivatives; 0/Buffers; 0/Calmodulin; 0/Free Radicals; 0/Oxidants; 0/Reactive Oxygen Species; 107-35-7/Taurine; 127-17-3/Pyruvic Acid; 7722-84-1/Hydrogen Peroxide; 80-15-9/cumene hydroperoxide; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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