| Sensitivity of porcine peripheral blood leukocytes to gamma irradiation in vivo, in vitro and ex vivo. | |
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MedLine Citation:
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PMID: 21250931 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: The large white pig is a useful experimental model to compare in vivo, in vitro and ex vivo sensitivity of peripheral blood leukocytes to ionising radiation. Such studies are impossible to perform in humans and laboratory rodents due to ethical reasons and body size, respectively. We analysed dose- and time-dependent changes of lymphocyte and granulocyte absolute numbers in porcine peripheral blood after either whole-body irradiation (in vivo and ex vivo experiments) or exposure of porcine whole blood to γ-irradiation (in vitro experiments). Materials and methods: CytoCount™ absolute counting beads and light scatter analysis using a flow cytometer were used to determine major leukocyte subpopulation numbers in blood samples after red cell removal. Results: Similar to other species, lymphocyte numbers significantly decreased in pigs both in vivo and in vitro in a dose-dependent manner. Most importantly, our data clearly show that reduction of lymphocyte numbers after irradiation in vivo proceeds much faster than after irradiation in vitro and that granulocyte changes depend only on the time of analysis after irradiation. Conclusions: All three tested experimental arrangements demonstrated the radiosensitivity of lymphocytes and the radioresistance of peripheral blood granulocytes. These in vivo and in vitro approaches, as well as the newly introduced ex vivo observations, appear to be relevant to biodosimetry. |
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Authors:
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Lenka Zarybnicka; Zuzana Sinkorova; Jiri Sinkora; Vaclav Blaha; Jirina Vavrova; Jaroslav Pejchal; Jan Osterreicher |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-21 |
Journal Detail:
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Title: International journal of radiation biology Volume: - ISSN: 1362-3095 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Trebesska. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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