| Sensitivity of morphological change of Vero cells exposed to lipophilic compounds and its mechanism. | |
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MedLine Citation:
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PMID: 20427127 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To find a sensitive cytotoxic response to reflect the toxicity of trace organic pollutants, the sensitivity and reliability of morphological change and proliferation inhibition of Vero cells exposed to lipophilic compounds and the leachate from products related to drinking water (PRDW) were compared, and the mechanism of the morphological change in Vero cells was studied. Results showed the proportion of morphologically changed cells increased with increasing 2,4,6-trichlorophenol (TCP)/perfluorooctane sulfonate (PFOS) concentration. However, at low TCP concentrations, inhibition of cell proliferation did not correlate to TCP concentration. After exposure to the leachate from PRDW extracted at different temperatures, the percentage of morphologically changed cells increased with extracting temperature, but the inhibition of cell proliferation failed to reflect the correlation to extracting temperature. These imply cell morphological change is a more sensitive and reliable method to reflect toxicity of trace organic pollutants than proliferation inhibition. Flow cytometry analysis indicated cell membrane damage was an early and sensitive cytotoxic response comparing with necrosis, resulting in cell morphological change, which may be due to the interference of lipophilic compounds. Lipophilic compound accumulated in cell membrane to interfere the assembly process of membrane protein and phospholipid. |
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Authors:
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Ting Ting Liao; Yan Ling Shi; Jian Wei Jia; Ru Wen Jia; Lei Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-02 |
Journal Detail:
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Title: Journal of hazardous materials Volume: 179 ISSN: 1873-3336 ISO Abbreviation: J. Hazard. Mater. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-05-14 Completed Date: 2010-08-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9422688 Medline TA: J Hazard Mater Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1055-64 Citation Subset: IM |
Copyright Information:
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2010 Elsevier B.V. All rights reserved. |
Affiliation:
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College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, China. liaotingting 82@163.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon / analysis Cell Membrane / drug effects, ultrastructure Cell Proliferation / drug effects Cell Shape / drug effects* Cercopithecus aethiops Chlorophenols / toxicity DNA / analysis, biosynthesis Environmental Pollutants / analysis, toxicity Flow Cytometry Lipids / chemistry, toxicity* Membrane Proteins / chemistry, metabolism Organic Chemicals / analysis, toxicity Phospholipids / analysis Tetrazolium Salts Thiazoles Vero Cells Water Pollutants, Chemical / chemistry, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Chlorophenols; 0/Environmental Pollutants; 0/Lipids; 0/Membrane Proteins; 0/Organic Chemicals; 0/Phospholipids; 0/Tetrazolium Salts; 0/Thiazoles; 0/Water Pollutants, Chemical; 298-93-1/thiazolyl blue; 7440-44-0/Carbon; 88-06-2/2,4,6-trichlorophenol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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