Document Detail

Sensitivity of morphological change of Vero cells exposed to lipophilic compounds and its mechanism.
MedLine Citation:
PMID:  20427127     Owner:  NLM     Status:  MEDLINE    
To find a sensitive cytotoxic response to reflect the toxicity of trace organic pollutants, the sensitivity and reliability of morphological change and proliferation inhibition of Vero cells exposed to lipophilic compounds and the leachate from products related to drinking water (PRDW) were compared, and the mechanism of the morphological change in Vero cells was studied. Results showed the proportion of morphologically changed cells increased with increasing 2,4,6-trichlorophenol (TCP)/perfluorooctane sulfonate (PFOS) concentration. However, at low TCP concentrations, inhibition of cell proliferation did not correlate to TCP concentration. After exposure to the leachate from PRDW extracted at different temperatures, the percentage of morphologically changed cells increased with extracting temperature, but the inhibition of cell proliferation failed to reflect the correlation to extracting temperature. These imply cell morphological change is a more sensitive and reliable method to reflect toxicity of trace organic pollutants than proliferation inhibition. Flow cytometry analysis indicated cell membrane damage was an early and sensitive cytotoxic response comparing with necrosis, resulting in cell morphological change, which may be due to the interference of lipophilic compounds. Lipophilic compound accumulated in cell membrane to interfere the assembly process of membrane protein and phospholipid.
Ting Ting Liao; Yan Ling Shi; Jian Wei Jia; Ru Wen Jia; Lei Wang
Related Documents :
20863677 - Cytotoxic action of bisabololoxide a of german chamomile on human leukemia k562 cells i...
20734917 - Cytotoxic compounds from poecilocerus pictus feeding on calotropis gigantea.
7621967 - Evaluation of cadmium chloride cytotoxicity in chinese hamster fibroblasts cultured in ...
20647067 - Mechanisms of cytotoxicity of asbestos fibres in rat tracheal epithelial cells in culture.
104477 - On the interactions of yersinia strains and cell cultures.
16736717 - Differentiation: an encouraging approach to anticancer therapy.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-02
Journal Detail:
Title:  Journal of hazardous materials     Volume:  179     ISSN:  1873-3336     ISO Abbreviation:  J. Hazard. Mater.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422688     Medline TA:  J Hazard Mater     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1055-64     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai, China. liaotingting
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carbon / analysis
Cell Membrane / drug effects,  ultrastructure
Cell Proliferation / drug effects
Cell Shape / drug effects*
Cercopithecus aethiops
Chlorophenols / toxicity
DNA / analysis,  biosynthesis
Environmental Pollutants / analysis,  toxicity
Flow Cytometry
Lipids / chemistry,  toxicity*
Membrane Proteins / chemistry,  metabolism
Organic Chemicals / analysis,  toxicity
Phospholipids / analysis
Tetrazolium Salts
Vero Cells
Water Pollutants, Chemical / chemistry,  toxicity*
Reg. No./Substance:
0/Chlorophenols; 0/Environmental Pollutants; 0/Lipids; 0/Membrane Proteins; 0/Organic Chemicals; 0/Phospholipids; 0/Tetrazolium Salts; 0/Thiazoles; 0/Water Pollutants, Chemical; 298-93-1/thiazolyl blue; 7440-44-0/Carbon; 88-06-2/2,4,6-trichlorophenol; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Measurement and source characteristics of carbonyl compounds in the atmosphere in Kaohsiung city, Ta...
Next Document:  Hydrothermal conversion of chrysotile asbestos using near supercritical conditions.