| Sensitivity of mitomycin-C treated McCoy cells for isolation of Chlamydia trachomatis from genital specimens. | |
| | |
MedLine Citation:
|
PMID: 3126059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new cell culture method was developed for the diagnostic isolation of Chlamydia trachomatis using McCoy cells pretreated with mitomycin-C. This drug acts on the cell by producing cross links between strands of DNA and results in the production of large, flat cells, which are similar to irradiated cells, and large chlamydial inclusions. In tests using a laboratory isolate of Chlamydia trachomatis, there were significantly more inclusions produced in mitomycin-treated monolayers than in cycloheximide-treated monolayers. Using clinical specimens, significantly more isolates were obtained in mitomycin-treated cells than in cycloheximide-treated cells. The new cell culture technique therefore offers a cell culture method which has the advantages of producing large inclusions similar to irradiated cells but with the convenience of drug treatment. |
| | |
Authors:
|
R M Woodland; R P Kirton; S Darougar |
Related Documents
:
|
11096419 - Computerized video time-lapse analysis of apoptosis of rec:myc cells x-irradiated in di... 12194309 - Development of the large scale digital cell analysis system. 13620889 - The effect of ultraviolet light on the sodium and potassium composition of resting yeas... 170989 - Uptake of [3h] thymidine and cell dna synthesis during the early multiplication phase o... 3117749 - Sodium butyrate stimulates cellular recovery from uv damage in xeroderma pigmentosum ce... 19393669 - Up-regulation of ros by mitochondria-dependent bystander signaling contributes to genot... 21600039 - Functional analysis of hoxd9 in human gliomas and glioma cancer stem cells. 1064679 - Uptake of adriamycin and daunomycin in l1210 and human leukemia cells: a comparative st... 12539779 - Probability of cell hits in selected organs and tissues by high-let particles at the is... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of clinical microbiology Volume: 6 ISSN: 0722-2211 ISO Abbreviation: Eur. J. Clin. Microbiol. Publication Date: 1987 Dec |
Date Detail:
|
Created Date: 1988-04-11 Completed Date: 1988-04-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8219582 Medline TA: Eur J Clin Microbiol Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 653-6 Citation Subset: IM |
Affiliation:
|
Section of Virology, Institute of Ophthalmology, London, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Line Cervix Uteri / microbiology Chlamydia Infections / diagnosis* Chlamydia trachomatis / isolation & purification* Cycloheximide / pharmacology Female Genitalia / microbiology* Humans Male Mitomycin Mitomycins / pharmacology* Rectum / microbiology Urethra / microbiology |
| Chemical | |
Reg. No./Substance:
|
0/Mitomycins; 50-07-7/Mitomycin; 66-81-9/Cycloheximide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Is alfentanil by infusion useful for sedation on the ITU?
Next Document: Interaction of the novel penem CGP 31 608 and its enantiomer with type Id beta-lactamase and penicil...