Document Detail

Sensitivity of magnetic resonance imaging of dendritic cells for in vivo tracking of cellular cancer vaccines.
MedLine Citation:
PMID:  17163419     Owner:  NLM     Status:  MEDLINE    
Success of immunotherapy with dendritic cells (DC) to treat cancer is highly dependent on their interaction with and activation of antigen specific T cells. To maximize DC-T cell contact accurate delivery of the therapeutic cells into the lymph node, or efficient trafficking of DC to the lymph nodes of the patient is essential. Since responses are seen in some patients but not in others, monitoring of the injected cells may be of major importance. Tracking of cells with magnetic resonance (MR) imaging is a non-invasive method that provides detailed anatomical information and is therefore more informative for the evaluation of the localization of therapeutic cells after injection than e.g. scintigraphic imaging. To challenge the sensitivity of this novel technique, we investigated the minimum amount of label and the number of cells required for MR imaging and the effect of labeling on DC function. DC were labeled with different concentrations of a clinically approved MR contrast agent consisting of superparamagnetic iron oxide particles and were imaged at both 3 and 7 T. Our results demonstrate the following: (i) When loaded with 30 (+/-4) pg Fe/cell, cell numbers as low as 1,000 cells/mm3 at 3 T and 500 cells/mm3 at 7 T could be readily imaged; (ii) Labeling does not affect cell viability and function; (iii) Because of its high spatial resolution and sensitivity, MRI is ideally suited to track therapeutic cells in vivo.
Pauline Verdijk; Tom W J Scheenen; W Joost Lesterhuis; Giulio Gambarota; Andor A Veltien; Piotr Walczak; Nicole M Scharenborg; Jeff W M Bulte; Cornelis J A Punt; Arend Heerschap; Carl G Figdor; I Jolanda M de Vries
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  120     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-01-25     Completed Date:  2007-03-13     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  978-84     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Department of Tumorimmunology, Nijmegen Centre for Molecular Life Science, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
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MeSH Terms
Cancer Vaccines / immunology*
Cell Movement*
Coculture Techniques
Contrast Media / analysis
Dendritic Cells / immunology*
Iron / analysis
Lymphocyte Activation
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles
Monocytes / immunology
Oxides / analysis
Sensitivity and Specificity
T-Lymphocytes / immunology
Grant Support
Reg. No./Substance:
0/Cancer Vaccines; 0/Contrast Media; 0/Magnetite Nanoparticles; 0/Oxides; 1317-61-9/Ferumoxytol; 7439-89-6/Iron; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides

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