Document Detail

Sensitivity of the fetal rat pituitary-adrenal system to corticotropin-releasing factor in organ culture.
MedLine Citation:
PMID:  1414217     Owner:  NLM     Status:  MEDLINE    
Six groups of adrenal glands from 17-day fetal rats were explanted to organ culture for 2 days. In one group, adrenal gland was cultured alone, and in the remaining five groups adrenal gland was cultured with pituitaries from fetuses ranging in age from 14 to 18 days. In each of the groups, half of the cultures had corticotropin-releasing factor (CRF) added to the medium. A histometric parameter utilized the size of adrenocortical cells as an indicator of sensitivity of the pituitary-adrenal system. When 17-day adrenal gland was cultured alone, addition of CRF did not cause any enlargement of cortical cells. When the adrenal gland was cultured with two 14-day pituitaries, cortical cells were enlarged. Addition of CRF to this culture induced no further change. With two 15-day pituitaries in the presence of CRF, cortical cells were slightly larger than those in the absence of CRF. With 16- to 18-day pituitaries, a marked hypertrophy of cortical cells was induced, and the addition of CRF caused further acceleration in their enlargement. These results suggest that, in organ culture, 14-day pituitary can release some adrenocorticotropic hormone (ACTH) with or without additional CRF. Older pituitaries (16- to 18-day) can apparently release an amount of ACTH in the presence of CRF that is greater than their own spontaneous ACTH secretion.
M Yamamoto; K Arishima; T Takizawa; M Shirai; Y Eguchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta anatomica     Volume:  145     ISSN:  0001-5180     ISO Abbreviation:  Acta Anat (Basel)     Publication Date:  1992  
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2007-07-02    
Medline Journal Info:
Nlm Unique ID:  0370272     Medline TA:  Acta Anat (Basel)     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  88-92     Citation Subset:  IM    
Department of Anatomy II, Azabu University School of Veterinary Medicine, Sagamihara, Japan.
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MeSH Terms
Adrenal Glands / cytology,  drug effects*,  embryology*
Cell Count
Corticotropin-Releasing Hormone / pharmacology*
Organ Culture Techniques
Pituitary Gland / cytology,  drug effects*,  embryology*
Rats, Wistar
Reg. No./Substance:
9015-71-8/Corticotropin-Releasing Hormone

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