| Sensitivity of an early step in the sporulation of Bacillus subtilis to selective inhibition by ethidium bromide. | |
| | |
MedLine Citation:
|
PMID: 4209775 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
When a final concentration of 0.4 mug of ethidium bromide (EB) per ml, which is subinhibitory to vegetative growth, is added to sporulating cells of Bacillus subtilis Marburg during either stage 0 or the early part of stage 1, morphogenesis is blocked. If the given concentration of EB is added after the early part of stage 1, sporogenesis is unaffected. The synthesis of the serine protease and antibiotic, which are believed to be associated with sporulation events during the early part of stage 0, are not inhibited by EB. Enhanced binding of [(14)C]benzylpenicillin to sporulating cells during septation (stage 2) is a measure of the presence of terminal enzymes for germ cell wall peptidoglycan synthesis. EB does not interfere with the binding of penicillin to sporulating cells, but penicillin remains more permanently bound to EB-treated postlogarithmic cells than to untreated sporulating cells. The absence of an interval of increased penicillin binding activity during stage 2 by sporulating cells treated with EB indicates that EB blocks sporulation prior to the completion of the germ cell wall. |
| | |
Authors:
|
M Rogolsky; H T Nakamura |
Related Documents
:
|
16662485 - Cell surfaces in plant-microorganism interactions : iii. in vivo effect of ethylene on ... 4012515 - Nutritional studies on paracoccidioides brasiliensis: the role of organic sulfur in dim... 332075 - Morphological changes in yeasts as a result of the action of 5-fluorocytosine. 11251855 - Chs1 of candida albicans is an essential chitin synthase required for synthesis of the ... 14656995 - Cell entry and antimicrobial properties of eukaryotic cell-penetrating peptides. 17533645 - Proteome and cytoskeleton responses in osteosarcoma cells with reduced oxphos activity. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of bacteriology Volume: 119 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1974 Jul |
Date Detail:
|
Created Date: 1974-09-11 Completed Date: 1974-09-11 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 57-61 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
biosynthesis Bacillus subtilis / drug effects*, enzymology, growth & development, metabolism Carbon Radioisotopes Cell Wall / metabolism Cell-Free System Endopeptidases / biosynthesis Ethidium / pharmacology* Morphogenesis / drug effects Penicillin G / metabolism Penicillin Resistance Peptidoglycan / biosynthesis Protein Binding Serine Spores, Bacterial / drug effects*, enzymology, growth & development, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Carbon Radioisotopes; 0/Peptidoglycan; 3546-21-2/Ethidium; 56-45-1/Serine; 61-33-6/Penicillin G; EC 3.4.-/Endopeptidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thymineless death in Bacillus subtilis: correlation between cell lysis and deoxyribonucleic acid bre...
Next Document: Adenine nucleotide changes associated with the initiation of sporulation in Bacillus subtilis.