Document Detail

Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation.
MedLine Citation:
PMID:  2172319     Owner:  NLM     Status:  MEDLINE    
The sensitivity of digoxigenin and biotin labelled DNA probes for the detection of human papillomavirus (HPV) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. Probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and detected with streptavidin, both methods having a common final visualisation procedure using alkaline phosphatase. Digoxigenin labelled probes proved two to 10-fold more sensitive by quantitative dot blotting and four-fold more sensitive in detecting HPV 16 DNA in a series of 31 anal carcinomas, compared with biotinylated probes. The digoxigenin method also produced less non-specific background staining of tissue sections than biotin labelled probes. It is concluded that digoxigenin DNA labelling and detection provides a simple, reliable, and efficient alternative to the use of biotin or radioactive isotopes for the detection of HPV DNA by in situ hybridisation. Digoxigenin labelled probes also offer the possibility of double labelling in situ hybridisation procedures when used with biotin labelled probes to provide simultaneous identification of different DNA sequences.
R G Morris; M J Arends; P E Bishop; K Sizer; E Duvall; C C Bird
Related Documents :
8078779 - Directly labeled dna probes using fluorescent nucleotides with different length linkers.
10707949 - In-situ detection of aspergillus fumigatus.
2538059 - Detection of viral genomes in the liver by in situ hybridization using 35s-, bromodeoxy...
9573819 - Identification of bacterial species on or in crevicular epithelial cells from healthy a...
1474979 - Development of a dna probe for ureaplasma urealyticum.
17318519 - Fast and sensitive dna analysis using changes in the fret signals of molecular beacons ...
9662609 - Detection of microbial pathogens in shellfish with multiplex pcr.
8414979 - How much hydration is necessary for the stabilisation of dna-duplex?
21135129 - Protein phosphatases pph3, ptc2, and ptc3 play redundant roles in dna double-strand bre...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  43     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-12-19     Completed Date:  1990-12-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  800-5     Citation Subset:  AIM; IM    
Department of Pathology, University Medical School, Edinburgh.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anus Neoplasms / microbiology
Biotin / diagnostic use*
Blotting, Southern
DNA Probes, HPV*
Digoxigenin / diagnostic use*
Genital Neoplasms, Female / microbiology
Genital Neoplasms, Male / microbiology
Laryngeal Neoplasms / microbiology
Nucleic Acid Hybridization*
Papillomaviridae / isolation & purification*
Tumor Virus Infections / diagnosis*
Uterine Cervical Neoplasms / microbiology
Reg. No./Substance:
0/DNA Probes, HPV; 1672-46-4/Digoxigenin; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Ultrasonographic difficulties and pitfalls in diagnosing primary carcinoma of the gallbladder.
Next Document:  In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.