Document Detail


Sensitivity of chronically HIV-1 infected HeLa cells to electrical stimulation.
MedLine Citation:
PMID:  12908087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Use of combination anti-retroviral drug regimens including protease inhibitors dramatically decreased morbidity and mortality rates in HIV-1 infected individuals. However, such combination therapies appear to have many side effects, in addition to the emergence of resistant HIV-1 strains. Therefore, in this study we sought to elucidate novel therapeutic principles against HIV-1 infection. We examined the effects of electrical stimulation on both chronically HIV-1(LAI) infected HeLa cells (P6 HeLa/HIV-1(LAI)) and uninfected cells (P6 HeLa). Cells were cultured on an optically transparent electrode and application of potential at 1.0 V vs Ag/AgCl was performed over time periods ranging from 10 min to 60 min. Both P6 HeLa/HIV-1(LAI) and P6 HeLa cells were progressively damaged as the duration of electrical stimulation increased. However, P6 HeLa/HIV-1(LAl) cells were much more influenced by electrical stimulation than P6 HeLa cells. The difference in damage rate was most obvious at 30 min of electrical stimulation, with damaged cells accounting for about 87% and 4% of P6 HeLa/HIV-1(LAI) and P6 HeLa cells, respectively. After the application of potential for 20 min, the proliferation of P6 HeLa/HIV-1(LAI) cells was markedly inhibited, while the P6 HeLa cells proliferated to an extent similar to that of uninfected cells without application of potential. These results indicate that sensitivity to electrical stimulation is much higher in chronically HIV-1 infected cells than in uninfected cells. This could be considered as a useful new approach against HIV-1 infection.
Authors:
E Kumagai; M Tominaga; S Harada
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Publication Detail:
Type:  Journal Article     Date:  2003-08-08
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  63     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-03-01     Completed Date:  2004-05-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  754-8     Citation Subset:  IM    
Affiliation:
Kumamoto University College of Medical Science, 4-24-1 Kuhonji, 862-0976, Kumamoto, Japan. etsu@cms.kumamoto-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Cell Division
Cell Physiological Phenomena*
Cell Size
Cell Survival
Electric Stimulation*
Electrodes
HIV Core Protein p24 / analysis
HIV-1 / physiology*
Hela Cells
Humans
Trypan Blue / metabolism
Chemical
Reg. No./Substance:
0/HIV Core Protein p24; 72-57-1/Trypan Blue

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