Document Detail

Sensitivity of bald eagle (Haliaeetus leucocephalus) hepatocyte cultures to induction of cytochrome P4501A by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
MedLine Citation:
PMID:  12739865     Owner:  NLM     Status:  MEDLINE    
Graded doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were added to primary hepatocyte cultures of bald eagle (Haliaeetus leucocephalus) embryos to determine their sensitivity to induction of cytochrome P4501A (CYP1A) and porphyrin accumulation. No porphyrin accumulation was observed, but both CYP1A catalytic activity (using the ethoxyresorufin-O-deethylase (EROD) assay) and immunodetectable CYP1A were induced by relatively high concentrations of TCDD. Bald eagle hepatocytes were less sensitive to CYP1A induction than hepatocytes from any other avian species that we have studied to date. These in vitro results are in general agreement with recent assessments of field data, which indicate that bald eagles are relatively insensitive to some of the effects of TCDD and related compounds. Preparation of bald eagle hepatocytes was challenging because existing methods did not yield monolayers of cells. Here we describe details of a new method that was successful for bald eagle hepatocytes. This new method is used routinely in our laboratory to prepare hepatocyte cultures from birds for examination of various biochemical responses to environmental contaminants.
Sean W Kennedy; Stephanie P Jones; John E Elliott
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Ecotoxicology (London, England)     Volume:  12     ISSN:  0963-9292     ISO Abbreviation:  Ecotoxicology     Publication Date:    2003 Feb-Aug
Date Detail:
Created Date:  2003-05-12     Completed Date:  2003-06-18     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9885956     Medline TA:  Ecotoxicology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  163-70     Citation Subset:  IM    
Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottowa, Ontario, Canada K1A 0H3.
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MeSH Terms
Cell Culture Techniques / methods
Cytochrome P-450 CYP1A1 / biosynthesis*,  pharmacology
Embryo, Nonmammalian
Environmental Pollutants / adverse effects*
Enzyme Induction
Hepatocytes / drug effects,  physiology*
Porphyrins / pharmacokinetics
Tetrachlorodibenzodioxin / adverse effects*
Reg. No./Substance:
0/Environmental Pollutants; 0/Porphyrins; 1746-01-6/Tetrachlorodibenzodioxin; EC P-450 CYP1A1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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