| Sensitivity of FISH in detection of MLL translocations. | |
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MedLine Citation:
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PMID: 10959098 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence in situ hybridisation (FISH) detection of MLL translocations is now commonplace in cytogenetics laboratories. One of the most widely used probes is the Oncor MLL probe (Oncor, Gaithersburg, MD) that theoretically demonstrates the presence of an MLL rearrangement by a splitting of the FISH signal between the two derivative chromosomes generated by a translocation. Recently, another commercial probe has been made available from Vysis (Vysis, Downers Grove, IL) that uses a dual colour system. We examined material from 29 patients and 4 cell lines, all with recognised MLL translocations by G-banding, that were confirmed using Southern blot analysis of the MLL breakpoint cluster region. Both Oncor and Vysis MLL FISH probes were applied to these cases to compare their performance in detection of the MLL translocations. Thirty of the 33 cases demonstrated a clear splitting of Oncor MLL FISH signal in concordance with the Southern blot analysis and cytogenetics. Three cases failed to demonstrate a split MLL FISH signal. Therefore, we conclude that the Oncor MLL FISH probe has a 9.1% false negative rate, i.e., 90.9% sensitivity in detection of classic MLL translocations. Vysis MLL FISH detected the rearrangement in all 33 cases. |
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Authors:
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G Cuthbert; K Thompson; G Breese; S McCullough; N Bown |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Genes, chromosomes & cancer Volume: 29 ISSN: 1045-2257 ISO Abbreviation: Genes Chromosomes Cancer Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-10-11 Completed Date: 2000-10-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9007329 Medline TA: Genes Chromosomes Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 180-5 Citation Subset: IM |
Copyright Information:
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Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
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School of Biochemistry and Genetics, University of Newcastle-upon-Tyne, United Kingdom. g.d.cuthbert@ncl.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Southern DNA Probes / genetics Female Fluorescent Dyes GTP Phosphohydrolases* GTP-Binding Proteins / analysis*, genetics* Genetic Variation / genetics Humans In Situ Hybridization, Fluorescence / methods* Sensitivity and Specificity Sequence Deletion / genetics Translocation, Genetic / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Fluorescent Dyes; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.1.-/SEPT9 protein, human |
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