Document Detail


Sensitivity of Candida albicans biofilm cells grown on denture acrylic to antifungal proteins and chlorhexidine.
MedLine Citation:
PMID:  19249746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Candida albicans cells form biofilms on polymeric surfaces of dentures and other prostheses introduced into the oral cavity. Many biofilm microorganisms exhibit resistance to antimicrobial agents; C. albicans cells may also develop resistance to naturally occurring antifungal peptides in human saliva including histatins (Hsts) and defensins (hBDs). Therefore, we evaluated Hst 5 activity on C. albicans biofilm cells compared to planktonic cells and measured whether surface treatment of denture acrylic with Hst 5, hBD-3, or chlorhexidine gluconate could inhibit in vitro biofilm development.
METHODS: Acrylic disks were preconditioned with 500 microl saliva for 30 min, and inoculated with C. albicans cells (10(6)cells/ml) for 1h, at 37 degrees C. Non-adherent cells were removed by washing and disks and were incubated in YPD growth medium for 24, 48, and 72 h at 37 degrees C. Candidacidal assays were performed on 48-h-biofilms and on planktonically grown cells using Hst 5 (15.5, 31.25, and 62 microM). Cell adhesion was compared on disks pre-coated with 0.12% chlorhexidine gluconate, 50 microM Hst 5, or 0.6 microM hBD-3 after 24, 48, and 72 h growth.
RESULTS: No significant difference was observed in sensitivity to Hst 5 of biofilm cells compared to planktonic cells (p>0.05). Pre-coating disks with hBD-3 did not inhibit biofilm development; however, Hst 5 significantly inhibited biofilm development at 72 h, while 0.12% chlorhexidine significantly inhibited biofilm development at all time intervals (p<0.05).
CONCLUSIONS: C. albicans biofilm cells grown on denture acrylic are sensitive to killing by Hst 5. Surface coating acrylic with chlorhexidine or Hst 5 effectively inhibits biofilm growth and has potential therapeutic application.
Authors:
Christopher R Pusateri; Edward A Monaco; Mira Edgerton
Related Documents :
7290266 - Action of misonidazole on l5178y-r and l5178y-s cells. i. comparison with antimycin a a...
20976076 - Investigation of antiangiogenic tumor therapy potential of microencapsulated hek293 veg...
15562586 - Inhibition of human embryonic palatal mesenchymal cell cycle by secalonic acid d: a pro...
9231006 - Effects of photodynamic therapy on glioma spheroids.
24706886 - Mathematical approaches to modeling development and reprogramming.
12909626 - The orc1 cycle in human cells: ii. dynamic changes in the human orc complex during the ...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2009-02-27
Journal Detail:
Title:  Archives of oral biology     Volume:  54     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2010-04-16     Revised Date:  2013-06-02    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  588-94     Citation Subset:  D; IM    
Affiliation:
Department of Restorative Dentistry, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY 14214, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acrylic Resins / chemistry*
Anti-Infective Agents, Local / pharmacology*
Antifungal Agents / pharmacology*
Biofilms / drug effects*,  growth & development
Candida albicans / drug effects*,  growth & development
Cell Adhesion / drug effects
Chlorhexidine / analogs & derivatives*,  pharmacology
Colony Count, Microbial
Dental Materials / chemistry*
Denture Bases / microbiology*
Histatins / pharmacology
Humans
Materials Testing
Polymethyl Methacrylate / chemistry
Saliva / physiology
Surface Properties
Temperature
Time Factors
beta-Defensins / pharmacology
Grant Support
ID/Acronym/Agency:
DE10641/DE/NIDCR NIH HHS; R01 DE010641-13/DE/NIDCR NIH HHS; R01 DE010641-14/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Acrylic Resins; 0/Anti-Infective Agents, Local; 0/Antifungal Agents; 0/Dental Materials; 0/HTN3 protein, human; 0/Histatins; 0/beta-Defensins; 0/beta-defensin 3, human; 53026-75-8/Lucitone; 55-56-1/Chlorhexidine; 9011-14-7/Polymethyl Methacrylate; MOR84MUD8E/chlorhexidine gluconate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A numerical tool for reproducing driver behaviour: Experiments and predictive simulations.
Next Document:  No evidence of attentional bias in obsessive compulsive checking on the dot probe paradigm.