Document Detail

Sensitivity of Candida albicans biofilm cells grown on denture acrylic to antifungal proteins and chlorhexidine.
MedLine Citation:
PMID:  19249746     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Candida albicans cells form biofilms on polymeric surfaces of dentures and other prostheses introduced into the oral cavity. Many biofilm microorganisms exhibit resistance to antimicrobial agents; C. albicans cells may also develop resistance to naturally occurring antifungal peptides in human saliva including histatins (Hsts) and defensins (hBDs). Therefore, we evaluated Hst 5 activity on C. albicans biofilm cells compared to planktonic cells and measured whether surface treatment of denture acrylic with Hst 5, hBD-3, or chlorhexidine gluconate could inhibit in vitro biofilm development.
METHODS: Acrylic disks were preconditioned with 500 microl saliva for 30 min, and inoculated with C. albicans cells (10(6)cells/ml) for 1h, at 37 degrees C. Non-adherent cells were removed by washing and disks and were incubated in YPD growth medium for 24, 48, and 72 h at 37 degrees C. Candidacidal assays were performed on 48-h-biofilms and on planktonically grown cells using Hst 5 (15.5, 31.25, and 62 microM). Cell adhesion was compared on disks pre-coated with 0.12% chlorhexidine gluconate, 50 microM Hst 5, or 0.6 microM hBD-3 after 24, 48, and 72 h growth.
RESULTS: No significant difference was observed in sensitivity to Hst 5 of biofilm cells compared to planktonic cells (p>0.05). Pre-coating disks with hBD-3 did not inhibit biofilm development; however, Hst 5 significantly inhibited biofilm development at 72 h, while 0.12% chlorhexidine significantly inhibited biofilm development at all time intervals (p<0.05).
CONCLUSIONS: C. albicans biofilm cells grown on denture acrylic are sensitive to killing by Hst 5. Surface coating acrylic with chlorhexidine or Hst 5 effectively inhibits biofilm growth and has potential therapeutic application.
Christopher R Pusateri; Edward A Monaco; Mira Edgerton
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2009-02-27
Journal Detail:
Title:  Archives of oral biology     Volume:  54     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2010-04-16     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  588-94     Citation Subset:  D; IM    
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MeSH Terms
Acrylic Resins / chemistry*
Anti-Infective Agents, Local / pharmacology*
Antifungal Agents / pharmacology*
Biofilms / drug effects*,  growth & development
Candida albicans / drug effects*,  growth & development
Cell Adhesion / drug effects
Chlorhexidine / analogs & derivatives*,  pharmacology
Colony Count, Microbial
Dental Materials / chemistry*
Denture Bases / microbiology*
Histatins / pharmacology
Materials Testing
Polymethyl Methacrylate / chemistry
Saliva / physiology
Surface Properties
Time Factors
beta-Defensins / pharmacology
Grant Support
Reg. No./Substance:
0/Acrylic Resins; 0/Anti-Infective Agents, Local; 0/Antifungal Agents; 0/Dental Materials; 0/HTN3 protein, human; 0/Histatins; 0/beta-Defensins; 0/beta-defensin 3, human; 53026-75-8/Lucitone; 9011-14-7/Polymethyl Methacrylate; MOR84MUD8E/chlorhexidine gluconate; R4KO0DY52L/Chlorhexidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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