Document Detail


Sensitive time-resolved fluoroimmunoassay for the detection of hazelnut (Corylus avellana) protein traces in food matrices.
MedLine Citation:
PMID:  16870207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Allergy to hazelnut is one of the most prevalent causes of severe food-allergic reactions in Norway, as recorded by The Norwegian National Reporting System and Register of Severe Allergic Reactions to Food. In the majority of the reported cases, there is "hidden", unlabelled hazelnut protein in processed foods like chocolate, cookies and cereal mixtures the eliciting agent. For a food survey study performed to evaluate the labelling practices with regards to hazelnut on behalf of the Norwegian Food Safety Authority, a new sensitive time-resolved fluoroimmunoassay (TR-FIA) for the detection of hazelnut protein traces in food matrices was developed and validated. The unique fluorometric properties of the europium-chelates used improved the signal-to-noise ratio because of low matrix interference and led to an enhanced sensitivity. The limit of detection was 0.1 mg/kg and the limit of quantitation was 0.33 mg/kg hazelnut protein. The recovery ranged from 73% to 123% in cookies and cereals, and from 50 to 77% in chocolate. The intra-assay precision was 7% and the inter-assay precision was 19%. Of 100 randomly chosen retail food products in Norway labelled "may contain hazelnut", 36 contained <0.2 mg/kg and seven >10 mg/kg hazelnut protein, demonstrating how differently this precautionary label is used.
Authors:
C K Faeste; L Holden; C Plassen; B Almli
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-10
Journal Detail:
Title:  Journal of immunological methods     Volume:  314     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-15     Completed Date:  2006-10-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  114-22     Citation Subset:  IM    
Affiliation:
National Veterinary Institute, PO BOX 8156,dep., N-0033 Oslo, Norway. christiane.faste@vetinst.no <christiane.faste@vetinst.no>
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MeSH Terms
Descriptor/Qualifier:
Corylus / adverse effects,  chemistry*
Fluoroimmunoassay / methods*,  standards
Food Analysis / methods*
Food Contamination / analysis*
Immunoblotting
Lanthanoid Series Elements / chemistry
Nut Hypersensitivity*
Plant Proteins / chemistry*,  isolation & purification
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Lanthanoid Series Elements; 0/Plant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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