| Sensitive spectrophotometric method for the quantitative estimation of collagen. | |
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MedLine Citation:
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PMID: 4083486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 micrograms protein. The method is specific, sensitive, simple, and rapid. |
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Authors:
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M Marotta; G Martino |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 150 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1986-02-07 Completed Date: 1986-02-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 86-90 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acids Azo Compounds* Chemical Precipitation Collagen / analysis* Coloring Agents* Spectrophotometry / methods |
| Chemical | |
Reg. No./Substance:
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0/Acetic Acids; 0/Azo Compounds; 0/Coloring Agents; 25188-41-4/C.I. direct red 80; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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