Document Detail

Sensitive spectrophotometric method for the quantitative estimation of collagen.
MedLine Citation:
PMID:  4083486     Owner:  NLM     Status:  MEDLINE    
A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 micrograms protein. The method is specific, sensitive, simple, and rapid.
M Marotta; G Martino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  150     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-02-07     Completed Date:  1986-02-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  86-90     Citation Subset:  IM    
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MeSH Terms
Acetic Acids
Azo Compounds*
Chemical Precipitation
Collagen / analysis*
Coloring Agents*
Spectrophotometry / methods
Reg. No./Substance:
0/Acetic Acids; 0/Azo Compounds; 0/Coloring Agents; 25188-41-4/C.I. direct red 80; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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