| Sensitive and specific quantification of sirolimus (rapamycin) and its metabolites in blood of kidney graft recipients by HPLC/electrospray-mass spectrometry. | |
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MedLine Citation:
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PMID: 8787698 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sirolimus (rapamycin) has a macrolide structure and is under clinical investigation as an immunosuppressant after organ transplantation. An HPLC/mass spectrometry assay to quantify sirolimus in blood was developed. 28-O-Acetyl sirolimus was used as internal standard. Blood samples were extracted with C18 columns. The extracts were injected into an HPLC system and isocratically eluted with methanol/1% formic acid (90/10 by vol) from a 150 X 4 mm C18 analytical column. The HPLC system was connected to a triple-stage quadrupole mass spectrometer with an electrospray interface and positive ions were detected. The limit of quantification in 1 mL of blood was 0.25 microgram/L and the calibration curve in blood was linear up to 250 microgram/L. The recovery from blood was 88 +/- 26% and interassay variation at 1 microgram/L was 19% and at 15 microgram/L 9.3%. Hydroxy, dihydroxy, demethyl, and didemethyl sirolimus as well as sirolimus were detected in blood of kidney graft patients. |
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Authors:
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F Streit; U Christians; H M Schiebel; K L Napoli; L Ernst; A Linck; B D Kahan; K F Sewing |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 42 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1996-10-22 Completed Date: 1996-10-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1417-25 Citation Subset: IM |
Affiliation:
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Institut für Allgemeine Pharmakologie, Medizinische Hochschule Hannover, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Anhydrides Chromatography, High Pressure Liquid / methods Drug Monitoring / methods* Humans Immunosuppressive Agents / blood* Kidney Transplantation* Mass Spectrometry / methods Molecular Structure Polyenes / blood* Sensitivity and Specificity Sirolimus |
| Chemical | |
Reg. No./Substance:
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0/Acetic Anhydrides; 0/Immunosuppressive Agents; 0/Polyenes; 108-24-7/acetic anhydride; 53123-88-9/Sirolimus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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