Document Detail

Sensitive radioimmunological screening test for antithyroglobulin autoantibodies.
MedLine Citation:
PMID:  627059     Owner:  NLM     Status:  MEDLINE    
The test described here was designed as a screening test to be used in conjunction with the radioimmunoassay for human thyroglobulin. Anti-thyroglobulin autoantibodies, when present even in low concentrations, interfere with thyroglobulin determination. A 30-min incubation allows binding of 125I-thyroglobulin to endogenous anti-thyroglobulin autoantibodies. A subsequent 2-h incubation with goat anti-human gamma-globulin causes precipitation of such complexes. The immunoprecipitable radioactivity reflects the binding capacity of the sample for 125I-labeled thyroglobulin. Samples that are devoid of autoantibodies, and are therefore suitable for valid thyroglobulin determinations, bind less than 6% of the radiolabeled thyroglobulin. The sensitivity of this radioimmunoassay exceeds that of the tanned-erythrocyte hemagglutination test.
P Bodlaender; J R Arjonilla; S L Twomey
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  24     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1978 Feb 
Date Detail:
Created Date:  1978-04-26     Completed Date:  1978-04-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  272-4     Citation Subset:  IM    
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MeSH Terms
Autoantibodies / analysis*
Blood Proteins / metabolism
Hemagglutination Tests
Iodine Radioisotopes / diagnostic use
Protein Binding
Radioimmunoassay / methods
Thyroglobulin / analysis,  blood,  immunology*
Thyroid Diseases / blood,  immunology
Reg. No./Substance:
0/Autoantibodies; 0/Blood Proteins; 0/Iodine Radioisotopes; 9010-34-8/Thyroglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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