| Sensitive immunoassay of human chorionic gonadotrophin based on multi-walled carbon nanotube-chitosan matrix. | |
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MedLine Citation:
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PMID: 18324418 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel amperometric immunosensor for human chorionic gonadotropin (HCG) assay has been fabricated through incorporating toluidine blue (TB) and hemoglobin (Hb) on the multiwall carbon nanotube (MWNT)-chitosan (CS) modified glassy carbon electrode, followed by electrostatic adsorption of a conducting gold nanoparticles (nanogold) film as sensing interface. The MWNT-CS matrix provided a congenial microenvironment for the immobilization of biomolecules and promoted the electron transfer to enhance the sensitivity of the immunosensor. Due to the strong electrocatalytic properties of Hb and MWNT toward H(2)O(2), the Hb and MWNT significantly amplified the current signal of the antigen-antibody reaction. The immobilized toluidine blue as an electron transfer mediator exhibited excellent electrochemical redox property. After the immunosensor was incubated with HCG solution, the access of activity center of the Hb to toluidine blue was partly inhibited, which leaded to a linear decrease in the catalytic efficiency of the Hb to the oxidation of immobilized toluidine blue by H(2)O(2) over HCG concentration ranges from 0.8 to 500 mIU/mL. Under optimal condition, the detection limit for the HCG immunoassay was 0.3 mIU/mL estimated at a signal-to-noise ratio of 3. Moreover, the proposed immunosensor displayed a satisfactory stability and reproducibility. |
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Authors:
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Na Li; Ruo Yuan; Yaqin Chai; Shihong Chen; Haizhen An |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-07 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 31 ISSN: 1615-7591 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-01 Completed Date: 2008-11-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 551-8 Citation Subset: IM |
Affiliation:
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Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, China. lina1019@swu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques Catalysis Chitosan / chemistry* Chorionic Gonadotropin / chemistry* Electric Impedance Electrochemistry / methods Electrodes Hemoglobins / chemistry Humans Hydrogen Peroxide / chemistry Immunoassay / instrumentation, methods* Microscopy, Electron, Scanning Nanotubes, Carbon / chemistry* Oxygen / chemistry Tolonium Chloride / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Hemoglobins; 0/Nanotubes, Carbon; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; 9012-76-4/Chitosan; 92-31-9/Tolonium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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