Document Detail


Sensitive enzyme immunoassay for human Cu/Zn superoxide dismutase.
MedLine Citation:
PMID:  2138526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A highly sensitive enzyme immunoassay method for measurement of human Cu/Zn superoxide dismutase (SOD) was established. Antisera were raised in rabbits by injecting SOD purified from human erythrocytes, and antibodies to SOD were purified by the use of Cu/Zn SOD-coupled Sepharose 4B. The assay system consisted of polystyrene balls with immobilized monospecific antibody F(ab')2 fragments and the same antibody Fab' fragments labeled with beta-D-galactosidase from Escherichia coli. The assay was highly sensitive, and the minimum detection limit was 3 pg or 0.1 fmol/assay tube. Serum Cu/Zn SOD levels of normal healthy subjects (36.3 +/- 15.6, n = 120, 16-64 years old) were not related to age and sex. It was confirmed that Cu/Zn SOD levels in erythrocytes and blood plasma were significantly enhanced in patients with Down syndrome. Immunoreactive Cu/Zn SOD was detectable in all the tissues examined and was present at high concentrations in brain, adrenal gland, liver, heart and kidney.
Authors:
N Kurobe; F Suzuki; K Okajima; K Kato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  187     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-05-02     Completed Date:  1990-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  11-20     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Aichi Prefectural Colony, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Down Syndrome / blood,  enzymology*
Erythrocytes / enzymology*
Female
Humans
Immunoassay / methods
Male
Middle Aged
Reference Values
Superoxide Dismutase / analysis,  blood*
Chemical
Reg. No./Substance:
EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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