Document Detail


Sensitive determination of gentiopicroside in medicine and bio-fluids using luminol-myoglobin chemiluminescence combined with flow injection technique.
MedLine Citation:
PMID:  19743525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel chemiluminescence method for the determination of gentiopicroside is presented, which was based on the inhibitory effect of gentiopicroside on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement of chemiluminescence intensity was linear with the logarithm of gentiopicroside concentration over the range from 10.0 pg mL(-1) to 500.0 ng mL(-1) (r(2) = 0.9992), with a detection limit of 3.0 pg mL(-1) (3σ). At a flow rate of 2.0 mL min(-1), a complete analytical process could be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n = 5). The proposed procedure was applied successfully in the determination of gentiopicroside in pharmaceutical preparations, human urine and serum without any pretreatment procedure. The possible mechanism of the reaction was also discussed.
Authors:
Xili He; Xiaofeng Xie; Xiaodong Shao; Zhenghua Song
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  25     ISSN:  1522-7243     ISO Abbreviation:  Luminescence     Publication Date:    2010 Sep-Oct
Date Detail:
Created Date:  2010-10-27     Completed Date:  2011-02-09     Revised Date:  2013-01-24    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  England    
Other Details:
Languages:  eng     Pagination:  384-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2009 John Wiley & Sons, Ltd.
Affiliation:
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, China.
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MeSH Terms
Descriptor/Qualifier:
Flow Injection Analysis* / instrumentation
Humans
Iridoid Glucosides / analysis*
Luminescence*
Luminescent Measurements / instrumentation
Luminol / chemistry*
Molecular Conformation
Myoglobin / chemistry*
Pharmaceutical Preparations / chemistry*
Chemical
Reg. No./Substance:
0/Iridoid Glucosides; 0/Myoglobin; 0/Pharmaceutical Preparations; 0WE09Z21RC/gentiopicroside; 521-31-3/Luminol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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