| Sensitive detection of phosphopeptides by matrix-assisted laser desorption/ionization mass spectrometry: use of alkylphosphonic acids as matrix additives. | |
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MedLine Citation:
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PMID: 18335463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been one of the most powerful tools for analyzing protein phosphorylation. However, it is frequently difficult to detect phosphopeptides with high sensitivity by MALDI-MS. In our investigation of matrix/matrix-additive substances for improving the phosphopeptide ion response in MALDI-MS, we found that the addition of low-concentration alkylphosphonic acid to the matrix/analyte solution significantly enhanced the signal of phosphopeptides. In this study, the combination of methanediphosphonic acid and 2,5-dihydroxybenzoic acid gave the best results. In addition to enhancing the signal of the phosphopeptides, alkylphosphonic acid almost completely eliminated the signals of sodium and potassium ion adducts. We report herein sensitive detection of phosphopeptides by MALDI-MS with the use of alkylphosphonic acids as matrix additives. |
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Authors:
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Hiroki Kuyama; Kazuhiro Sonomura; Osamu Nishimura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 22 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-03 Completed Date: 2008-06-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
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Languages: eng Pagination: 1109-16 Citation Subset: IM |
Affiliation:
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Institute for Protein Research, Osaka University, Suita, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diphosphonates / chemistry* Gentisates / chemistry* Indicators and Reagents Peptide Mapping* Phosphopeptides / chemistry* Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Diphosphonates; 0/Gentisates; 0/Indicators and Reagents; 0/Phosphopeptides; 1984-15-2/methylene diphosphonate; 490-79-9/2,5-dihydroxybenzoic acid |
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