Document Detail


Sensitive detection of anaerobic monochlorobenzene degradation using stable isotope tracers.
MedLine Citation:
PMID:  17612157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microbial degradation of monochlorobenzene (MCB) under anaerobic conditions was investigated using a stable isotope tracer under in and ex situ conditions. In situ microcosms were incubated directly in an anoxic aquifer and carbon derived from [13C6]-MCB was found to be incorporated into the microbial biomass. In laboratory microcosms, amended with [13C6]-MCB, anaerobic mineralization of MCB was indicated by the production of 13CO2. Further, recovery of the 13C-label in the fatty acids confirmed the assimilation of MCB-derived carbon into microbial biomass. The described approach may be applied to various other organic groundwater contaminants of concern using carbon (13C) as well as other stable isotope tracers, such as nitrogen (15N), allowing direct and sensitive detection of biodegradation.
Authors:
Ivonne Nijenhuis; Nicole Stelzer; Matthias Kästner; Hans-H Richnow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental science & technology     Volume:  41     ISSN:  0013-936X     ISO Abbreviation:  Environ. Sci. Technol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-07-06     Completed Date:  2007-08-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3836-42     Citation Subset:  IM    
Affiliation:
Department of Isotope Biogeochemistry (ISOBIO), UFZ Helmholtz Centre for Environmental Research, Permoserstrasse 15, 04318 Leipzig, Germany. ivonne.nijenhuis@ufz.de
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Anaerobic / metabolism*
Biodegradation, Environmental
Biomass
Carbon Isotopes
Chlorobenzenes / metabolism*
Water Pollutants, Chemical / metabolism*
Chemical
Reg. No./Substance:
0/Carbon Isotopes; 0/Chlorobenzenes; 0/Water Pollutants, Chemical; 108-90-7/chlorobenzene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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